Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Feed Review》 2011-12
Add to Favorite Get Latest Update

Study on Determination Method of Egg Yolk Cholesterol Content

ZHANG Xu,HU Yan,JIANG Guitao,WANG Xiangrong,LI Haobang,DAI Qiuzhong*(Hunan Institute of Animal Science and Veterinary Medicine,Changsha 410131,China)  
The essay contrasted and studied on three methods of determinating the egg yolk cholesterol content.The three methods were High performance liquid chromatography(HPLC),saponification-colorimetric method,and kit method.The results showed that HPLC method can accurately and fast analysis cholesterol content in egg yolk.The accuracy of HPLC was better than saponification-colorimetric method and kit method.The pre-treatment methods was using 95% ethanol dispose yolk diluent,then using ether and petroleum ether mixed extract cholesterol,afterwards blow dry with nitrogen and add anhydrous alcohol to made dissolve,filtrate with 0.45 μm organic microporous membrane filter,so the sample is made.Chromatographic conditions was using C18 column,acetonitrile-isopropanol(4:1,v/v) as mobile phase,flow velocity was 0.8 mL·min-1,column temperature was 35 ℃,detected wavelength was 210 nm,sample amount was 10 μL,the peak time was 14.578 min.Test used the external standard method quota.
【Fund】: 湖南省畜牧兽医研究所自选基金(ZR200901)
【CateGory Index】: S879.3
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 5 Hits
1 Zhang Jiacheng (Department of Food Science, Agricultural University of Hebei,Baoding, 071001) Luo Chengxiang (College of Food Science,Northeast Agricultural University,Herbin,150030) Huang Chinan (Administrative Office of Agriculture, Daqing City, D;Cholesterol Analysis in Foods by Direct Saponification colorimetric Method[J];FOOD AND FERMENTATION INDUSTRIES;2000-03
2 SHENG Ling-hui,WANG Jing,WU Li-qing,GAO Yun-hua,FU Bo-qiang,ZhAO Zheng-yi (National Institute of Metrology,Beijing 100013,China);Determination of Cholesterol Content in Egg Powder with High-performance Liquid Chromatography-Mass Spectrometry[J];Food Science;2008-12
3 GE Qing-lian1,GE Qing-feng2,LIU Di1,L Xiao-juan1,ZHU Yun-fen1,LIU Xue-xian1,CHEN Kuan-wei1 (1. Poultry Institute,Chinese Academy of Agricultural Sciences,Yangzhou 225003,China;2. College of Tourism Cuisine,Yangzhou University,Yangzhou 225002,China);Catalytic Spectrophotometry with Enzyme for Determination of Cholesterol in Eggs[J];Food Science;2009-02
4 TAN Guan-yue1, FU Hong1, YANG Lin2(1. College of Biological Science and Technology, Fuzhou University, Fuzhou 350002, Fujian, China;2. Monitoring Center of Marine Environment and fishery resource, Fuzhou 350003, Fujian, China);THE COMPARISON OF TWO METHODS FOR CHOLESTEROL DETERMINATION IN EEL BY HPLCAND CHOLESTEROL KIT[J];Food Research and Development;2008-04
5 Zhang Rongzhen, Li Long, Liu Shutao, Chen Ruming and Rao Pingfan (Institute of Biotechnology, Fuzhou University, Fuzhou, 350002);An Improved Method of Cholesterol Determination in Egg Yolk by High Performance Liquid Chromatography[J];CHINESE JOURNAL OF CHROMATOGRAPHY;1998-02
Chinese Journal Full-text Database 10 Hits
1 LU Qing-ping1 , ZHANG Hong-fu1 , FU Jing2, LIU Fu-zhu2 (1.Animal Science Institute,CAAS,Beijing 100094,China ; 2.Northwest Science Technique University of Agriculture and Forestry,Yangling 7 12100,China);EFFECT OF PAPRIKA EXTRACT SUPPLEMENT ON EGG YOLK COLOUR OF LAYER FED WHEAT-BASED DIETS[J];Acta Zoonutrimenta Sinica;2005-02
2 ZHANG Xu JIANG Guitao WANG Xiangrong HU Yan LI Haobang DAI Qiuzhong(Animal Nutrition and Feeding Technology Department,Hunan Institute of Animal Science and Veterinary Medicine,Changsha 410131,China);Effects of Tea Polyphenols on Performance,Egg Quality and Yolk Cholesterol Content of Laying Hens[J];Chinese Journal of Animal Nutrition;2011-05
3 Lü Jin-Shun*1, Wei Chang-Mei1, Xu Ji-Ming1, Zhu Hui2, Xie Zhong-Kui3 1(Huaiyin Teachers College, Jiangsu Key Laboratory for Chemistry of Low-Dimensional Materials, Huaian 223300) 2(Jiangxi Science and Technology Normal University, Nanchang 330013) 3(Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000);Feature Analysis of Structure of Potato Dietary Fibre[J];Chinese Journal of Analytical Chemistry;2007-03
4 ZOU Feng-ping1,2,XIONG Ming1,YU Qiong-wei*1,TANG Ziyong2,FENG Yu-qi1 (1.College of Chemistry and Molecular Sciences,Wuhan University,Wuhan 430072;2.Faculty of Material Science and Chemical Engineering,China University of Geosciences,Wuhan 430074);Determination of Cholesterol in Monocyte-derived Macrophages by High Performance Liquid Chromatography[J];Journal of Analytical Science;2007-06
5 HUANG Dao Ping CHEN Jian Hong LAO Yan Wen ZHOU Lin MO Jian Guang (Guangxi Research Centre of Analysis,No.32 Xinghulu,Nanning 530022,P.R.China );Determination of Cholesterol in Export Moon Cake by HPLC UV Detector[J];Chinese Journal of Spectroscopy Laboratory;2001-04
6 HE Chun-mei (Guangxi Agricultural Vocational and Technical Institute,Nanning 530007,China);Analysis of cholesterol in egg yolk by direct saponification-colorimetric method[J];Guangdong Agricultural Sciences;2011-09
7 DOU Weiwei1,HU Lizhi2,ZHU Xiuqing2*,CHEN Hao2,YU Dianyu1(1.Food College,Northeast Agricultural University,Harbin 150030,China;2.The National Research Centre of Soybean Engineering and Technology,Harbin 150050,China);Study on determination of sterol in transesterificational vegetable oil[J];Journal of Northeast Agricultural University;2011-08
8 XIA Suyin1 WANG Chengzhang1 ZHAN Fabai2 LIU Qishuan1 CHEN Yanrong1 LI Haiyan1(1.College of Animal Science and Veterinary Medicine,Henan Agricultural University,Zhengzhou 450002,China;2.Rural Economic Development Center of Luochen Township in Guangshan County,Guangshan 465436,China);Alfalfa Meal and Cellulase Affect Lipid Metabolism,Antioxidant Capacity and Intestinal Flora of Layers[J];Chinese Journal of Animal Nutrition;2011-11
9 YU Wen-jing1,ZHENG Li1,SHEN Chong-yu2(1.Suzhou Entry & Exit Inspection and Quarantine Bureau,Suzhou 215104,China; 2.Jiangsu Entry & Exit Inspection and Quarantine Bureau,Nanjing 210001,China);Test of Qualitative and Quantitative Method for Cholesterol in Hogwash Oil by LC/MS/MS[J];Journal of Chongqing University of Technology(Natural Science);2011-12
10 WU Zhong-ling1,HE Zhong-qiang1,GUO Mao-zhang1,CHEN Shu-dong1,TANG Wei-qiang2 (1.Guangzhou Quality Supervision and Testing Institute,National Centre for Quality Supervison and Testing of Processed Food,Guangzhou 510110,China;2.School of mechanical & automatic engineering,Guangzhou 510460,China);Determination of Cholesterol in Fat by Solid Phase Extraction—GC/MS[J];Packaging and Food Machinery;2011-06
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 LOU Hong-xing1,2, LIN Zi2, WANG You-ming3, LU Fu-zhuang1, TANG Jun-feng2, YING Jun-feng2, YANG Zhong-ming3 (1.Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 2.Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China; 3. Feed Research Institute,Zhejiang University, Hangzhou 310029, China);Effect of Dietary Tea Polyphenols on Performances, Lipid Metabolism and Egg Quality of Laying Hens[J];Journal of Tea Science;2004-02
2 WANG Shi-kui,TONG Jian-ming(Institute of Animal Science,CAAS,Beijing 100094,China);REVIEW IN MANIPULATION OF CHOLESTEROLMETABOLISM IN LAYING HENS[J];Acta Zoonutrimenta Sinica;2002-03
3 ZHANG Yong1,2, WANG Jing2, LIN Dong-kang3 (1.College of Biological Engineering,Henan University of Technology,Zhengzhou 450052,China; 2.Institute of Animal Science,CAAS Beijing 100094,China;3.Engineering College of Animal Husbandry and Veterinary Science,Henan Agricultural University,Zhengzhou 450002,China);EFFECTS OF ALFALFA GLYCOSIDES ON PERFORMANCE, SERUM LIPID AND CARCASS QUALITY IN BROILERS[J];Acta Zoonutrimenta Sinica;2005-04
4 HONG Wei~(1,3),GONG Yue-sheng ~(1),LI Ji-gang~3,YAN Wei-jun~(3)(1.College of Animal Science and Technology,Northwest A & F University,Yangling,Shaanxi,712100,China;2.Yangling Animal Husbandry and Veterinary Station,Yangling,Shaanxi,712100,China;3.Weinan Vocational Technical Collage,Weinan,Shaanxi,715501,China);Effects of Allicinon on Production Capacity Caecum Microorganism and Serum Biochemical Index in Laying Hens[J];Progress In Veterinary Medicine;2005-09
5 Lin Xianglin 1 Lin Fanping 2 ( 1Fujian Southeast Feed Co.Ltd, Fuzhou 350007; 2College of Animal Science, Fujian Agricultural University, Fuzhou 350002);Effects of organic chromium on the production performance and yolk cholesterol of laying hens[J];JOURNAL OF FUJIAN AGRICULTURAL UNIVERSITY(NATURAL SCIENCE);1999-04
6 YU Bi Yu LIU Xiang Long LU Xiao\|Long (Testing Center,Yangzhou University,Yangzhou,Jiangsu 225009,P.R.China) WANG Zheng\|Lin CHENG Yu\|Long (Yangzhou Import & Export Cammodity Imspection Bureau,Yangzhou,Jiangsu 225009,P.R.China);Determination of Total Cholesterol in Food by Enzyme Catalytic Spectrophotometry[J];CHINESE JOURNAL OF SPECTROSCOPY LABORATORY;1999-06
7 Tang Zhaoxin ( Anhui Agricultural Universit, Hefei, 230036 );Effects of Different Dosage of Ge-132 on Germanium Enrichment and Chotesterol Contents in Eggs[J];Heilongjiang Journal of Animal Science and Veterinary Medicine;1995-12
8 LIANG Xu Fang, BAI Jun Jie , LAO Hai Hua  , LI Gui Sheng, ZHOU Tian Hong, Hiroshi Y. Ogata  (Department of Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou, 510632)  (Key Lab. of Tropical & Subtropical Fish Breeding & Cultivation, Pear River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Ministry of Agriculture, Guangzhou, 510380)  (National Research Institute of Aquaculture, Nansei, Mie 516 0193, Japan);NUTRITIONAL REGULATION OF LIPOPROTEIN LIPASE GENE EXPRESSION AND VISCERAL FAT DEPOSITION IN RED SEA BREAM ( PAGRUS MAJOR )[J];Oceanologia Et Limnologia Sinica;2003-06
9 Liu Pingxiang Chen Lujiang Xie Debiao Xiong Xiaomei(College of Animal Science and Technology, JAU, Nanchang330045);Effects of Dietary Chromium on the Productivity of Laying Hens and the Distribution of Chromium[J];ACTA AGRICULTURAE UNIVERSITIS JIANGXIENSIS;1999-04
10 WO Xing De, CUI Xiao Qiang, and TANG Li Hua(Molecular Medical Institute of Zhejiang Traditional Chinese Medical College, Hangzhou 310053, China);Effects of Curcumin on the Activities of Plasma Lipase and the Concentrations of Lipids in Hyperlipidemic Rats Induced by High Lipid Diet[J];Chinese Journal of Arterial Lerosis;2003-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved