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Analysis of Fatty Acids Harpodon Nehereus in by GC/MS

SUN Li-yang,DIAO Quan-ping,TAO Jing(Department of Chemistry,Anshan Normal University,Anshan 114007,China)  
The contents of fatty acids in Harpodon nehereus were determined by capillary gas chromatography-mass spectrometry following Bligh-Dyer extraction and potassium hydroxide-methanol esterfication.The results showed that Bligh-Dyer extraction exhibits a high extraction efficiency.Unsaturated fatty acid are the main components in the fifteen kinds of fatty acids found in Harpodon nehereus,in which the relative content of 4,7,10,13,16,19-Docosahexaenoic acid methyl ester and 5,8,11,14,17-Eicosapentaenoic acid methyl ester is 9.37% and 5.55% respectively.
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