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Inhibitory effect of cholecystokinin-octapeptide on production of cytokines in the lung of endotoxic shock rats

MENG Ai Hong, LING Yi Ling *, ZHAO Xiao Yun, ZHANG Jun Lan, WANG Qiu Hong Department of Pathophysiology, Hebei Medical University, Shijiazhuang 050017  
To study the effect of cholecystokinin octapeptide (CCK 8) on systemic hypotension and cytokine production in serum and lung of endotoxic shock (ES) rats induced by lipopolysaccharide (LPS) and investigate its signal transduction mechanism of p38 mitogen activated protein kinase (MAPK), the changes in mean arterial pressure (MAP) were observed by using a polygraph in four groups of SD rats: group of LPS (8 mg/kg i v ) induced ES, group of CCK 8 (40 μg/kg i v ) pretreatment 10 min before LPS (8 mg/kg) administration, group of CCK 8 (40 μg/kg i v ) only , and normal saline (control) group; the contents of proinflammatory cytokines (TNF α, IL 1β and IL 6) in the lung and serum were assayed using ELISA kits; and p38 MAPK was detected by Western blot. The results showed that CCK 8 alleviated LPS induced decrease in MAP of rats; compared with the control, LPS elevated the levels of TNF α, IL 1β and IL 6 in serum and lung significantly, while CCK 8 significantly inhibited the LPS induced increases in TNF α, IL 1β and IL 6 in serum and lung. The activation of p38 MAPK in the lung of ES rats was enhanced by CCK 8 pretreatment. These results suggest that CCK 8 can alleviate the LPS induced decrease in MAP of ES rats and exert an inhibitory effect on the overproduction of proinflammatory cytokines, and that p38 MAPK may be involved in its signal transduction mechanisms.
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