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《Acta Physiological Sinica》 2003-02
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Migration and differentiation of human bone marrow mesenchymal stem cells in the rat brain

HOU Ling-Ling 1, ZHENG Min 1, WANG Dong-Mei 1, YUAN Hong-Feng 1, LI Hai-Min 1, CHEN Lin 1, BAI Ci-Xian 1, ZHANG Yong 2, PEI Xue-Tao 1,* 1Beijing Institute of Transfusion Medicine, Beijing 100850; 2Northwest Sci-Tech University of Agriculture and Forestry, Yangling, Shanxi 712100  
Bone marrow mesenchymal stem cells (MSCs) are multipotent tissue stem cells that can be induced in vitro to differentiate into a variety of cells such as osteoblasts, chondrocytes and adipocytes. MSCs are useful vehicles for both cell and gene therapy for a variety of diseases. Here, we injected human MSCs with enhanced green fluorescent protein (EGFP) into the striatum of Parkinson disease (PD) rat and examined their survival, migration, differentiation, and the behavior changes in PD rats, which will provide a theoretical foundation and technical method for clinic PD therapy by stem cells. The results showed that human bone marrow MSCs can survive in rat brain for a long time (exceeding 70 d). MSCs were found in multiple areas of the rat brain including the striatum, the corpus callosum, contralateral cortex and even the brain vascular wall. Immunocytochemical staining suggested that implanted cells expressed human neurofilament (NF), neuron-specific enolase (NSE) and glial fibrillary acid protein (GFAP). At the same time, remission in abnormal behavior of the PD rats appeared. Rotation scores decreased gradually from 8 86±2 09 r/min pre-transplantation to 4 87±2 06 r/min 90 d post-transplantation (statistic result showed P0 05).
【Fund】: Thisworkwassupportedbythegrantsfrom 863Program ( #2 0 0 2AA2 0 5 0 5 1and #2 0 0 1AA2 1615 1)ofMinistryofScienceandTechnologyofChina from973Program( #2 0 0 1CB5 0 9906)ofMinistryofScienceandTechnologyofChina andfromBeijingInnovationProject( #A0 2 0 2
【CateGory Index】: R329
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