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《Life Science Research》 2018-01
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Transcriptome of Boleophthalmus pectinirostris Skin and Analysis of Antimicrobial Peptide Related Unigenes

ZHANG Yu-xia;SHI Ge;WANG Ri-xin;GONG Yan-bin;FAN Mei-hua;LIAO Zhi;Laboratory of Marine Biology Protein Engineering, Marine Science and Technical College, Zhejiang Ocean University;Marine Science College, Ningbo University;  
Antimicrobial peptides(AMPs) are employed by fish as the innate defense peptides to protect against the invasion of foreign microorganisms and are important lead molecules for the development of novel drugs.Boleophthalmus pectinirostris is a special fish that can live in amphibious environment; and the mucus from its skin is speculated to be rich in AMPs and has great significance to its immune defense and adaptation to the amphibious life. For understanding the gene expression patterns of B. pectinirostris skin and screening for antibacterial peptide related genes, the transcriptome analysis of the adult B. pectinirostris skin was performed by Illumina sequencing. A total of 78 608 366 paired-end reads amounting to 6 GB of sequence data were generated and further de novo assembled with Trinity software, yielding a total of 119 848 high-quality skin unigenes. When these unigenes were annotated in the available databases, 7 unigenes were found to en-code peptides homologous with the reported fish AMPs of 5 families with significant hits(E-value1.00 E-05),i.e. β-defensin, hepcidin, NK-lysin, piscidin, and liver-expressed antimicrobial peptide-2(LEAP-2). Their differential expression in different tissues, sequence alignment and phylogenetic analysis were performed. The results provide a foundation for further understanding the immune defense mechanism for B. pectinirostris to adapt to the amphibious environment and developing novel antibiotics using the newly identified AMPs.
【Fund】: 国家自然科学基金资助助项目(31472279)
【CateGory Index】: S917.4
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