Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Life Science Research》 2018-01
Add to Favorite Get Latest Update

Construction of Human LDHA Gene Promoter Plasmid and Analysis of Its Transcriptional Regulation and Function by Nrf2 in Lung Cancer Cells

CAO Yu-xiang;ZHOU Xing-lu;LI Xiang-rui;HUANG Tao;KE Li-xia;WU Zhi-hao;College of Life Science, Anhui Normal University;College of Medical Imaging and Laboratory,Wannan Medical College;Clinical Medical College,Wannan Medical College;Department of Biology, School of Basic Medicine,Wannan Medical College;  
In order to investigate the effect of nuclear factor erythroid 2-related factor 2(Nrf2) on tumor metabolism and, consequently, tumor migration, we focused on regulation of lactate dehydrogenase A(LDHA) ex-pression by Nrf2. Herein, the plasmid LDHA-luc containing the firefly luciferase reporter gene driven by human LDHA gene promoter was successfully constructed, and transfected into A549 cells together with Nrf2 expresion vector. After transfection, the dual luciferase reporter gene assay system showed that the LD-HA promoter activity in cells transfected with Nrf2 plasmid was significantly higher than that of the control.Meanwhile, the expression level of LDHA in cells transfected with Nrf2 plasmid was also higher, indicating that Nrf2 promoted transcription and expression of LDHA in A549 cells. Up-regulation of LDHA promotes the formation of acidic tumor microenvironment and cell migration. Therefore, successful construction of the plasmid LDHA-luc and demonstration of LDHA gene expression regulation by Nrf2 lay a foundation for further research on how Nrf2 influences tumor metabolism to promote cell migration through LDHA.
【Fund】: 国家自然科学基金面上项目(81272359);; 皖南医学院高层次引进人才启动基金
【CateGory Index】: R734.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved