Mitochondrial DNA Content Affects the Fertilizability of the Improved Triploid Crucian Carp
XU Wen-ting;ZHOU Rong;HU Xin-jiang;XIAO Ya-mei;PENG Liang-yue;State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University;School of Life Sciences, Hunan Normal University;Yutan Middle School;
Improved triploid crucian carp is a hybrid polyploid fish(red crucian carp(♀) × allotetraploid carp()). Its infertility properties make it valuable for production and research. Previous studies have shown that mitochondria play an important role in infertility of animals, but there are no relevant reports in fish. Herein,the unique fish lines in our laboratory(including improved triploid crucian carp, red crucian carp, allotetraploid carp and autotriploid crucian carp), which have clear genetic backgrounds, were compared by fluorescence quantitative PCR. The results showed that mitochondrial DNA(mt DNA) content in testis and ovary of improved triploid crucian carp was higher and lower, respectively, than that in fertile individuals. It was the first time to explore the relationship between mitochondria and infertilizability of improved triploid crucian carp from the perspective of mt DNA content affecting germ cell development. The study provides a new explanation for the molecular mechanism of infertilizability in improved triploid crucian carp.