Effects of different hypoxia on proliferation of dermal scar fibroblasts
LI Gao-feng, TAN Jun, ZHONG Qian, et al. (Department of Plastic and Cosmetic Surgery, Hunan Province People′s Hospital, Changsha 410005, China)
Objective To explore the effects of different hypoxia on proliferation of dermal scar fibroblasts. Methods Dermal scar fibroblasts in vitro cultured for 3～4 days were placed in an incubator with levels at 20%, 10%, 5%, 1.5% O_2 and were removed at 24?h intervals for study. Cell proliferation was determined by cell counting and PCNA expression by immunohistochemical staining. Results With the O_2 concentrations decreasing, dermal scar fibroblast cell counting was increased and the expressions of PCNA were strengthened gradually. Conclusions Hypoxia can induce dermal scar fibroblast proliferation. Decreasing hypoxia may be advantageous to prevent scar proliferation.
【CateGory Index】： R622