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《Chinese Journal of Industrial Medicine》 2005-01
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A study on biological effects of low intensity pulsed ultrasound on cultured cells

KONG Lu,YANG Hong,ZHAO Jin-shun (School of Public Health,Southeast University,Nanjing 210009,China)  
Objective To study the biological effect of low-intensity pulsed ultrasound(LPU)on the cultured cell in vitro.Method The biological effects of LPU on the proliferation of human skin fibroblasts were determined by MTT assay.The cell apoptosis of human skin fibroblasts and MiaPaCa-2 cells was determined with Annexin Ⅴ assay.Result (1)Optical absorption value of cell activity in MTT assay increased significantly in the cell culture with LPU treatment for 6,11 and 20 minutes without FCS,as compared with the control group(P0.01),with a peak detected in the cell culture with LPU treatment for 11 minutes.Optical absorption value also increased in the cell culture with 5% FCS,and statistical significance(P0.05,P0.01).(2)Cell proliferation was inhibited and cell apoptosis increased significantly in human skin fibroblasts and MiaPaCa-2 cells with LPU stimulation for more than 60 minutes(P0.01),as compared with the control group.Conclusion Stimulation with LPU could induce cell proliferation at a shorter time and could inhibit cell growth or even cause cell apoptosis in vitro at a longer time.
【Fund】: 东南大学国家自然科学基金预研项目 (Y2 0 0 32 51 34)
【CateGory Index】: Q813
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Chinese Journal Full-text Database 2 Hits
1 MENG Xian-mei1,2,LU Shi-ying2,YAN Dong-ming2,LIU Zeng-shan2(1.Biological Engineering Department,Jilin Business and Technology College,Changchun 130062;2.Key Laboratory for Zoonoses Research,Ministry of Education,Jilin University,Changchun 130062);Progress of saxitoxin research and application[J];Food Science and Technology;2010-08
2 HUANG Hai-yan,FU Ying-bin,ZHAO Kun-shan,YUAN Jian-hui,ZHOU Li,LIU Jian-jun(Shenzhen Center for Disease Control and Prevention,Shenzhen 518020,China);In vitro evaluation of paralytic shellfish poisoning toxin potency[J];Chinese Journal of Health Laboratory Technology;2010-04
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