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《Journal of Food Safety & Quality》 2014-12
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Study on viability of multiple drug-resistant and sensitive Escherichia coli in vitro gastrointestinal environment

ZHANG Mei;LIU Xiao-Yang;LI Kai-Xiong;JI Hua;LU Shi-Ling;LUO Juan;ZHOU Hong;College of Food Science and Technology,Shihezi University;  
Objective Escherichia coli is human and warm blooded animal intestinal important facultative anaerobic microflora group. It is indicator bacteria of water and food fecal pollution. The viability of multiple drug-resistant and drug sensitive Escherichia coli in vitro gastrointestinal environment was studied. The results provided a theoretical basis for control of harmful Escherichia coli. Methods The 30 retail food were collected from supermarkets of Kuitun City, the 8 positive samples were identified. The contamination rate was 27%. Then the disk diffusion method was used to conduct the drug test for 8 E.coli strains isolated from 8 positive samples respectively. A multi-drug resistant E.coli E27 and a drug sensitive E.coli E25 were implemented in artificial gastric juice and the intestinal juice by tolerance test. Results The results show that the measured eight E.coli were sensitive to most drugs. The resistant bacteria were keeping resistance for ampicillin and tetracycline. There were four drug-resistant bacteria and four sensitive bacteria. Conclusion The tolerance was good for E.coli E25 in vitro gastrointestinal juice. And the tolerance was not high for E.coli E27 in vitro gastrointestinal juice. The decreasing trend of sensitive bacteria E25 was slower than multi-drug resistant bacteria E27.
【Fund】: 国家自然科学基金项目(31301469、31160329、31360392);; 石河子大学高层次人才启动项目(RCZX201225);; 石河子大学青年骨干教师项目(3152SPXY02033);; 国家星火计划项目(2013GA891006);; 新疆生产建设兵团科技攻关项目(2013BA012)~~
【CateGory Index】: R446.5
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