Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Food and Fermentation Industries》 2005-08
Add to Favorite Get Latest Update

A Novel Micro-amount Method with Spectrophotometer to Determine Amylose and Total Starch Content of Wheat Seeds

Zhao Yongliang (Key Biotech. Lab, Institute of Crops Science, Chinese Agricultural Academy of Science and Technology, Beijing, 100081, China)  
The amylose and total starch content are important parameters which determined wheat quality as well as the health function of wheat. Quick, simple and minimal-quantity determinations of amylose and total starch contents is required in selecting and breeding of excellent quality wheat and processing research. A novel micro-amount method with spectrophotometer(MAS) was discovered on the basis of national standard GB7648—1987 with some improvements. Determination results of amylose content showed that parallel errors of 10 wheat samples using the MAS were less than 2% of 10mg samples by using non-degreasing and basal dissolution at 65℃ for 12 h; the errors between MAS and GB7648—1987 method were also less than 2%; both kinds of errors were accepted according to GB7648—1987. The total starch content was also determined at the same time using the standard curve of water-solvable starch by MAS, the errors of two parallel experiments in group and between groups of MAS and GB are less than 1%, which were also permitted according to GB. The results indicated that MAS is characterized with needing micro-amount sample(1/10 of GB), no need of degreasing, lower temperature, lower pollution, saving time and labor, quick and acuteness, quality-repetition, and easy batch-determination.
【Fund】: 国家高技术研究发展计划“863”项目(No.2001AA211031)
【CateGory Index】: TS211
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Wang guangpeng1,2 Liu qingxiang2 Kong dejun2 Zhang zhihong1* (1 horticulture College of Shenyang Agricultural University,shenyang,110160;2 Fruit Institute,Hebei Academic of Agriculture and Forestry Sciences,Changli,066600);Comparative Studies on two Methods for Analyzing starch Content in Castanea mollissima BL.[J];Anhui Agricultural Science Bulletin;2007-18
2 Tian Gaoyou 1 , Yuan Hongfu 1, Liu Huiying 2, Lu Wanzhen *1 1(Research Institute of Petroleum Processing,Beijing, 100083) 2(Beijing POL Institute, Beijing, 102300);Application of Wavelet Transform on the Near Infrared Analysis[J];Chinese Journal of Analytical Chemistry;2004-09
3 Chu Xiaoli~ *1 , Tian Gaoyou~ 2 , Yuan Hongfu~ 1 , Lu Wanzhen~ 1 ~ 1 (Research Institute of Petroleum Processing, Beijing 100083) ~ 2 (Beijing POL Ititute, Beijing 102300);Quantitative Analysis of Near Infrared Spectroscopy by Combination of Wavelet Analysis and N-way Partial Least Square[J];Chinese Journal of Analytical Chemistry;2006-S1
4 Chu Xiaoli Wang Yanbin Lu Wanzhen (Research Institute of Petroleum Processing,Beijing,100083);Present situation and prospect of near infrared spectrometer in China and abroad.[J];Analytical Instrumentation;2007-04
5 YUE Jun-ming, CHEN Ying, DING Ying(Institute of Bio-technology, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China);Determination of Tobacco Chemical Components by Near Infrared Spectrum Method[J];Guizhou Agricultural Sciences;2005-03
6 ZHAO Chen, QU Hai-bin, CHENG Yi-yu Department of Chinese Medicine Science and Engineering, Zhejiang University, Hangzhou 310027, China;A New Approach to the Fast Measurement of Content of Amino Acids in Cordyceps Sinensis by ANN-NIR[J];Spectroscopy and Spectral Analysis;2004-01
7 WANG Duo-jia 1, 2, ZHOU Xiang-yang 1, JIN Tong-ming 1, HU Xiang-na 1, ZHONG Jiao-e 1, WU Qi-tang 2 1. Shenzhen Inspection Station of Non-pollution Agricultural Products, Shenzhen 518040, China 2. South China Agriculture University, Guangzhou 510000, China;Application of Near-Infrared Spectroscopy to Agriculture and Food Analysis[J];Spectroscopy and Spectral Analysis;2004-04
8 CHEN Da, WANG Fang, SHAO Xue guang, SU Qing de Department of Chemistry, University of Science and Technology of China, Hefei 230026, China;Research on the Nonlinear Model of Near Infrared Spectroscopy and the Total Sugar of Tobacco Samples[J];Spectroscopy and Spectral Analysis;2004-06
9 ZHENG Yong-mei 1, ZHANG Jun 2, CHEN Xing-dan 2, SHEN Xuan-guo 1, ZHANG Tie-qiang 1 1. Centre of Physics Teaching and Research, Jilin University, Changchun 130025, China 2. Changchun Institute of Optic, Fine Mechanics and Physics, Changchun 130022, China;The PLS Analysis with Short Wave Near-Infrared of Whole Wheat for Protein[J];Spectroscopy and Spectral Analysis;2004-09
10 TIAN Gao-you 1, YUAN Hong-fu 1, CHU Xiao-li 1, LIU Hui-ying 2, LU Wan-zhen 11. Research Institute of Petroleum Processing, Beijing 100083, China2. Beijing POL Institute, Beijing 102300, China;Near Infrared Spectra (NIR) Analysis of Octane Number by WaveletDenoising-Derivative Method[J];Spectroscopy and Spectral Analysis;2005-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved