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《Food and Fermentation Industries》 2005-08
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A Novel Micro-amount Method with Spectrophotometer to Determine Amylose and Total Starch Content of Wheat Seeds

Zhao Yongliang (Key Biotech. Lab, Institute of Crops Science, Chinese Agricultural Academy of Science and Technology, Beijing, 100081, China)  
The amylose and total starch content are important parameters which determined wheat quality as well as the health function of wheat. Quick, simple and minimal-quantity determinations of amylose and total starch contents is required in selecting and breeding of excellent quality wheat and processing research. A novel micro-amount method with spectrophotometer(MAS) was discovered on the basis of national standard GB7648—1987 with some improvements. Determination results of amylose content showed that parallel errors of 10 wheat samples using the MAS were less than 2% of 10mg samples by using non-degreasing and basal dissolution at 65℃ for 12 h; the errors between MAS and GB7648—1987 method were also less than 2%; both kinds of errors were accepted according to GB7648—1987. The total starch content was also determined at the same time using the standard curve of water-solvable starch by MAS, the errors of two parallel experiments in group and between groups of MAS and GB are less than 1%, which were also permitted according to GB. The results indicated that MAS is characterized with needing micro-amount sample(1/10 of GB), no need of degreasing, lower temperature, lower pollution, saving time and labor, quick and acuteness, quality-repetition, and easy batch-determination.
【Fund】: 国家高技术研究发展计划“863”项目(No.2001AA211031)
【CateGory Index】: TS211
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