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《Food and Fermentation Industries》 2006-06
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Screening and Optimizing of Lactobacillus bulgaricus and Streptococcus thermophilus Wort Enrichment Medium

Wan Hongbing Tian Hongtao Shan Lijie Sun Jilu Li Yingjun Sun Yongqiang(Food Science and Technology College,Agricultural University of Hebei,Baoding 071000,China)  
In this paper,effects of adding different nutriment in wort medium on the growth of Streptococcus thermophilus(S.t-3) were studied.Furthermore,the optimum enrichment medium of S.t-3 were screened by orthogonality test,then the growth situation of Lactobacillus bulgaricus(L.b-DR,L.b-S1) in the optimum medium was tested.The results showed that S.t-3,L.b-DR,L.b-S1 grew well in wort medium.Soya peptone,Beef Extract,Yeast Extract could promote the growth of S.t-3 significantly.For K_2HPO_4,it could not only promote bacteria's growth, but also protect lactobacillus from being damaged by low pH of medium.The optimum enrichment medium obtained by orthogonality test consist of adding 0.5% Soya peptone,0.5% Yeast Extract,1% Beef Extract,0.2% K_2HPO_4 in the 10% wort medium.The living number of S.t-3,L.b-DR,L.b-S1 cultivated for 16 hours in the optimum enrichment medium at 37℃ were 2.42×10~9 cfu/mL,2.85×10~9 cfu/mL,4.81×10~9 cfu/mL which was the same with the living number in the MRS used in lab,but the production cost was decreased 1 000 yuan/t compared with using MRS medium.
【Fund】: 河北省“十五”科技攻关专项专题:优良菌种选育及高效发酵剂的研制(No.03220171D)
【CateGory Index】: TS201.3
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