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Rapid Evaluating of Antimicrobial Activity of Vanillin with the Microplate Reader in 96-cell Plate

Chen Mo 1,2,Wang Zhiwei 1,2,Hu Changying 3,Wu Xiyang 3,Wang Pingli 2 1(Department of packaging Engineering,Jiangnan University,Wuxi 214122,China)2(Packaging Engineering Institute,Zhuhai College,Jinan University,Zhuhai 519070,China)3(Department of Food Science and Engineering,Jinan University,Guangzhou 510632,China)  
The Microplate Reader is used to detect the antimicrobial activity of vanillin against Escherichia coli,Staphylococcus aureus,Listeria monocytohenes,Salmonella enteritidis,and Shigella sonnei in a sterile 96-well microtitre plate.The growth curves of tested bacteria indicate that vanillin exhibits certain antimicrobial effect against E.coli,S.aureus,L.mono,S.enteritidis,and Sh.sonnei,with minimum inhibitory concentration(MIC)values of 1.6mg/mL,2.2 mg/mL,1.0 mg/mL,1.2 mg/mL and 1.6 mg/mL,respectively.This method is proved to be economical,rapid and effective for detecting the efficiency of antimicrobial.
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