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《Food and Fermentation Industries》 2011-12
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Detection of Biogenic Amines in Xuanwei Ham by HPLC

Liao Guo-zhou1,Wang Gui-ying2,Cao Jin-xuan3,Chen Zhi-bin4 1(College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China) 2(Department of Animal Husbandry and Veterinary,Yunnan Agricultural Vocational Technical College,Kunming 650212,China) 3(College of Life Science and Biotechnology,Ningbo University,Ningbo 315211,China) 4(College of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China)  
Biogenic amines content was investigated in Xuanwei ham.Samples were extracted with 0.4 mol/L HClO4,and then derived with dansyl chloride.Separation was achieved using ZORBAX XDB-C18 column and identification,and a quantitative analysis of biogenic amines fraction was carried out using a HPLC system with DAD.Results showed that the linear ranges were 0.5~20 μg/mL,the limit of detection was 0.05~0.1 μg/mL,the recoveries were 81.58%~95.58% and the relative standard deviation were 2.48%~5.85% for eight biogenic amines.Six biogenic amines were detected in Xuanwei ham,the content of β-phenylethylamine,putrescine,cadaverine,tyramine,spermidine and spermine were 0.56 mg/100g,1.45 mg/100g,0.37 mg/100g,3.62 mg/100g,1.26 mg/100g and 4.28 mg/100g,respectively.
【Fund】: 云南省教育厅研究基金项目(09C0262);; 云南省现代农业生猪产业技术体系专项(云财农[2011]191号)
【CateGory Index】: TS251.7;O657.72
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