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The Cellulase Production by Trichoderma reesei in Submerged Fermentation

Yu Xiaobin (School of Bioengineering,Wuxi University of Light Industry,Wuxi,214036) Koo Yoonmo (Dept. of Biological Engineering, Inha University, Inchon, Korea)  
By comparing cellulase activity of different strains, Trichoderma reesei Rut C-30 was found to be the best one. A combination of Avicel and wheat bran was used as carbon source, as well the KH 2PO 4-K 2HPO 4 buffer system was used for pH control, resulted in very high enzyme production in shake flask culture, Maximum CMCase 100-125U/ml, FPA 9-12U/ml were achieved within 6 days culture. When it was grown in 2.5L fermentor, where pH and DO levels were controlled, the enzyme activity were 133.4U/ml(CMCase)and 11.67U/ml(FPA),respectively. Dried cellulase powder was prepared by (NH 4) 2SO 4 precipitation method, showed 3074.9U/g of CMCase activity and 166.7U/g of FP activity.
【CateGory Index】: TQ925.9
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