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《The Food Industry》 2012-11
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Purification of Saponins from Gynostemma

Zhang Hong-mei College of Vocational and Technical,Inner Mongolia Agricultural University(Baotou 014109)  
Studied the extraction of saponins from Gynostemma in separation and purification processes.Ginseng leaf water extraction and alcohol precipitation,feed-water ratio 1:10,after 70% concentrated alcohol precipitation,polysaccharide protein was gained.Supernatant was concentrated and adsorbed by the D101,LSA-21 macroporous resin,washed to colorless.After 30%,50%,70%,90% ethanol eluting,the eluting solution was collected and bleached by Al2O3 column,washed to colorless by water,at last saponins was concentrated.Results showed that the content of total saponins of Gynostemma purified by D101 macroporous resin and eluted with 70% ethanol was the highest,the purification was 91.67%.Gynostemma saponin content in leaves was significantly higher than that in the roots,polysaccharide protein was also gained in the extraction while extracting saponins.
【CateGory Index】: TQ461
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