Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《The Food Industry》 2013-03
Add to Favorite Get Latest Update

Research on the Conditions Optimization for Laccase Production from Trametes Versicolor and the Enzymatic Characteristics on Laccas

Zhao Wen-juan1*,Xu Sheng-yun1,Ren Ping1 1.Enzyme Engineering Institute of Shaanxi Sciences(Xi’an 710600);2.Shaanxi Engineering Center for Enzyme Technology(Xi’an 710600)  
The constituent of culture medium and culture conditions for the trametes versicolor(mys 004) screened was studied and optimized.And its enzymatic characteristics were also researched.Its culture medium consisted of 20% potato juice 1 L(K2HPO4 1.0 g,NaCl 0.5 g,MgSO4.7H2O 1.5 g,NaNO3 2.5 g) CaC12.2H2O 0.1 g,FeC13 0.02 g,tween 80 1.2 mL,pollard 10 g,guaiacol 0.006%.The activity of the yielding laccase was 751 U/mL under the conditions of the culture pH 4.5,the culture temperature 28 ℃,the rotational speed of culture 200 r/min,the culture time 7 d.Its enzymatic characteristics was using the temperature 30~55 ℃(the optimum temperature 50 ℃),the optimum pH 4.5.The storage temperature could not exceed for 60 ℃,the optimum temperature less than 40 ℃.When there was 0.5 mmol/L Cu2+ or Mg2+,the enzymatic activity was not effected.When there was 5 mmol/L Zn2+,Mn2+,Co2+ or Al3+,the enzymatic activity was descended approximately for 50%.The enzymatic activity was descended approximately for 70% if the Fe3+ existed.The enzymatic activity entirely lost if the Fe2+ existed.
【Fund】: 陕西省科学院计划项目(2011K-11)
【CateGory Index】: TQ925
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 7 Hits
1 HOU Hongman1,2,JIANG Jiaojiao1(1. Coll. of Bio. & Food Technol., Dalian Inst. of Light Ind., Dalian 116034,China;2. School of Environ. Sci. and Eng., Dalian Univ. of Technol., Dalian 116012, China);Laccase production by white rot fungi Pleurotus ostreatus and its optimal inducing condition[J];Journal of Dalian Institute of Light Industry;2003-01
2 GUO Mei1, PU Jun2, LIANG Peng1, WEN Bin1, LU Fu-ping2, DU Lian-xiang2 (1.Department of Food Science,Tianjin Agriculture College,Tianjin 300384, China;2.Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300222,China);STUDIES ON FERMENTATION CONDITIONS FOR LACCASE PRODUCTION FROM WHITE-ROT FUNGI TRAMETES VERSICOLOR[J];Food Research and Development;2006-06
3 CAO Zhi-yun 1 ,ZHENG Teng 2,3 ,XIE Bi-feng 1 ,LU Cheng-ping 2 1.College of Bioengineering,Fujian Normal University,Fuzhou350007;2.College of Veterinary Medicine,Nanjing Agri-culture University,Nanjing210095;3.Inspection and Quarantine Technique Center of Fujian,Fuzhou350003;China;Advances of industrial application of laccase[J];Letters in Biotechnology;2004-04
4 WANG Yilei (Biology Department of Heze Teacher's college, Heze,Shandong 274015);Studies on the Properties of Laccase and Screening for High Yield Laccase Producing Fungi[J];Journal of Microbiology;2004-01
5 GUAN Yan-li1,HUA Shuang2,ZHAO Xin-hai1,ZHANG Qing-hua1,ZHONG Li-juan1,ZHU Wei-wei1,WU Hong-yan1(1.Liaoning Scientific Research Academy of Microbiology,Chaoyang 122000;2.Shenyang Agric.Uni.Shenyang 110161);Isolation,Purification and Enzymatic Features of Laccase from Trametes trogii[J];Journal of Microbiology;2011-04
6 MENG Qinghui1,CUI Yanhong2(1.College of Life Science and Engineering,Northwest University for Nationalities,Lanzhou 730030,China; 2.College of Animal Science,Henan Institute of Science and Technology,Xinxiang 453003,China);Optimization of low temperature enzymes production by Phanerochaete Chrysosprium and the enzyme properties[J];China Brewing;2009-09
7 Zhu Mingqi, Cao Zhimin, Li Zhenqi(College of Plant Protection, Northwest A & F University, Yangling Shaanxi 712100);Screening of High Laccase-Producing Strain from Trametes and Preliminary Study on Its Fermentation Conditions[J];Chinese Agricultural Science Bulletin;2006-03
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LIANG Shuai et al (College of Resources and Environment,Central South University of Forestry and Technology,Changsha,Hunan 410004);Research Progress and Application Prospect of Laccase from White Rot Fungus[J];Journal of Anhui Agricultural Sciences;2008-04
2 WANG Dan-mei et al (College of Biological Science and Technology,Shenyang Agriculture University,Shenyang,Liaoning 110161);Study on Fermentation Conditions of Pycnoporus Sanguineus[J];Journal of Anhui Agricultural Sciences;2008-16
3 LIU Qing-yu et al(Shenyang Agricultural University,Shenyang,Liaoning 110161);Experimental Study on the Effects of White-rot Fungus on the Production Ability of Methane with Corn Straw Stalk[J];Journal of Anhui Agricultural Sciences;2008-29
4 HU Xue-zhu(Nanyang Agricultural School,Nanyang,Henan 473000);Reach Advance of Ligninolytic Enzymes[J];Journal of Anhui Agricultural Sciences;2011-11
5 ZHANG Li-ping,GAO Hui(School of Forestry and Landscape Architecture,Anhui Agricultural University,Hefei 230036);Effects of different conditions on laccase activity of white-rot fungus[J];Journal of Anhui Agricultural University;2009-02
6 ZHAO Min;YANG Qian;XIN Ying;ZHU Shiqi;MU Qingfeng. Department of Life Science and Engineering, Harbin Institute of Technology,150001,P.R.China.;Induction and production of laccase in Elfvingia applanata.[J];Journal of Beijing Forestry University;2004-04
7 SONG Xiao-shuang~1; ZHAO Min~2; LIU Gui-feng~1; WANG Yu-cheng~1; YANG Qian~3.1 College of Forestry, Northeast Forestry University, Harbin, 150040, P. R. China; 2 College of Life Sciences, Northeast Forestry University, Harbin, 150040, P. R. China; 3 Department of Life Sciences and Engineering, Harbin Institute of Technology, 150010, P. R. China.;Cloning and sequence analysis of laccase gene fragments from Climacocystis borealis.[J];Journal of Beijing Forestry University;2005-05
8 HAN Xu;ZHAO Min;LU Lei.College of Life Science,Northeast Forestry University,Harbin,150040,P.R.China.;Optimization of bilirubin oxidase production from Myrothecium verrucaria and its application in dye decolorization[J];Journal of Beijing Forestry University;2010-04
9 GUO Mei1,LU Fu-ping2,LIU Min-yao3,LI Tuo-ping1,LIANG Peng1,PU Jun2(1.Department of Food Science,Tianjin Agriculture College,Tianjin 300384,China;2.Key Laboratory of Industrial Microbiology,College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300222,China;3.Tianjin Nuoao Technology Company,Tianjin 300384,China);Studies on the degradation of 2-chlomphenol by laccase-secreting engineered strain[J];Journal of Agricultural University of Hebei;2009-02
10 Jin Chunde,Wang Jin,Wu Wei,Wang Zhenming(School of Engineering,Zhejiang Forestry University,Lin'an 311300,P.R.China);Effects of Metal Ions and EDTA on ROS Free Radical Produced from Laccase-treated Bamboo Powder[J];Journal of Northeast Forestry University;2008-12
China Proceedings of conference Full-text Database 1 Hits
1 WANG De-han ,XIANG Qian-bin , CHEN Guang-yin (South China Agricultural University, Guangzhou, Guangdong 510642, China);Research Progress of Ecological Higher Value Application of Spent Mushroom Compost~1[A];[C];2007
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Hui et al (School of Life Science, Liaocheng University, Liaocheng,Shandong 252059);Research Advances in the Biodegradation of Lignin[J];Journal of Anhui Agricultural Sciences;2006-09
2 WANG Xi-wen, ZHAN Huai-yu, HE Wei( South China University of Technology, Guangzhou, Guandong 510640);Effect of metal ions on the laccase activity[J];China Pulp & Paper Industry;2003-06
3 Song Ruiqing, Yang Qian(Harbin Institute of Technology, Harbin 150001, P. R. China); Deng Xun(Northeast Forestry University);Degradation of Pleurotus ostreatus (Jeca ex Fr.) Quel. Strain to Straw[J];Journal of Northeast Forestry University;2004-01
4 HOU Hongman1,2,JIANG Jiaojiao1(1. Coll. of Bio. & Food Technol., Dalian Inst. of Light Ind., Dalian 116034,China;2. School of Environ. Sci. and Eng., Dalian Univ. of Technol., Dalian 116012, China);Laccase production by white rot fungi Pleurotus ostreatus and its optimal inducing condition[J];Journal of Dalian Institute of Light Industry;2003-01
5 HAN Xiao-lei, YAN Lian-he, ZHOU Shen-fan(Water Treatment Institute, Nanjing University of Science & Technology, Nanjing 210094,China);The Influences on the Production and Activity of Laccase:A Review[J];;2005-07
6 FANG Hua,HUANG Jun,CHEN Zhao,DING Li-yun(Fiber Optic Sensing Technology Research Center,Wuhan University of Technology,Wuhan 430070,China);Study on Fermentation Conditions for Producing Laccase of Pleurotus Ostreatus[J];Chemistry & Bioengineering;2008-08
7 ZHANG Li ping 1,DONG Chao 1,WANG Ying chun 1,GAN Shuan you 2,ZHAO Qian 1 CHENG Hui cai 1,JIN Qing sheng 1 (1.Institute of Biology, Hebei Academy of Sciences,Shijiazhuang 050081,China; 2.Hebei Grain School,Shijiazhuang 050051,China);Effect of some ions on cellulase activity[J];Journal of The Hebei Academy of Sciences;2000-04
8 GUO Wei-yun1,TANG Xiang-zhong2,YAO Zhao-yang3,SHAO Qiang4,LI Zong-yi4 (1.Department of Life Science and Technology,Xinxiang Medical College,Henan Xinxiang 453003,China;2.Henan Institute for the Control of Medical Device,Henan Zhengzhou 450008,China;3.School of Basic Medicine,Xinxiang Medical College,Henan Xinxiang 453003,China;4.College of Life Science,Henan Normal University,Henan Xinxiang 453007,China);Purification and Characterization of Isozymes of Laccase from Trametes versicolor[J];Journal of Hebei Normal University(Natural Science Edition);2007-06
9 JIANG Ling1,WU Haizhen1,WEI Chaohai2,LIANG Shizhong1(1 School of Bioscience and Bioengineering,South China University of Technology,Guangzhou 510640,Guangdong,China;2 College of Enviroment Science and Engineering,South China University of Technology,Guangzhou 510640,Guangdong,China);Research progress of characterization of white-rot fungus enzyme system for lignin degradation and its application[J];Chemical Industry and Engineering Progress;2007-02
10 Huang Danlian Zeng Guangming Hu Tianjue Huang Guohe Chen Yaoning (Department of Environmental Science and Engineering, Hunan University, Changsha 410082);Preliminary study on the application of white-rot fungus in composting of lignin waste[J];Techniques and Equipment for Environmental Pollution Control;2005-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved