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《Food & Machinery》 2011-05
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Study on antibacterial activity and stability of extracts from skin of bufo bu fo

WANG Yuan-qing1,2 YAN Jian-ye2 LUO Kun2 SHI Bai-mei2(1.Biotechnology Department,The College of Life Science And Technology,Central South University ofForestry and Technology,Changsha,Hunan410004,China;2.Key Lab of Modernization of ChineseMedicine,School of Pharmacy,Hunan University of Chinese Medicine,Changsha,Hunan410208,China)  
Paper disc method was used to observe the antibacterial ac-tivity of extracts from the skin of bufo bufo with different solvents on tested strains.The minimal inhibitory concentration(MIC)was test-ed by plate two-fold dilution method.And the dose-effect relation-ship and the heat-pressure stability of the antimicrobial active frac-tion of the skin of bufo bufo were also studied.The results showed that the water extracts,30%alcohol extracts and ethanol sediments from the skin of bufo bufo had no bacteriostasis activities on the tested strains.The 60%ethanol extracts and the 90%ethanol extracts had bacteriostasis activities on the tested strains inordinately and the 90%ethanol extracts was better than the 60%ethanol extracts.The MIC of 90%ethanol extracts to Eschrichia coli,Staphyl-ococcus aureus,Bacillus subtilis and Aspergillus niger were 1 000 mg/mL,500mg/mL,500 mg/mL,500 mg/mL,respectively.There were dose-effects relationship between the inhibitory effect and total alkaloid,and the antimicrobial active fraction had heat-pressure stability.
【Fund】: 湖南省教育厅科学研究项目资助(编号:11C1321);; 中南林业科技大学青年科学研究基金资助(编号:2009015B);; 湖南省高校“中药新药创制与资源综合持续利用”科技创新团队资助(编号:湘教通[2010]212号)
【CateGory Index】: TQ461
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