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《Food & Machinery》 2016-05
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Response surface methodology for optimization of extracting polysaccharide from Polygala tenuifolia willd.and antioxidant evaluation

JING Yong-shuai;WU Lan-fang;WANG Qian;ZHENG Yu-guang;YAN Yu-ping;JIANG Ai-min;College of Chemistry and Pharmaceutical Engineering,Hebei University of Science and Technology;College of Pharmacology,Hebei University of Chinese Medicine;College of Food Science,South China Agricultural University;  
The optimum condition of ultrasonic extraction of Polygala tenuifolia Willd.Polysaccharide(PTP)was investigated.The results showed,the optimum condition was ultrasonic extraction time30 min,ultrasonic extraction,temperature 67 ℃,ratio of water to raw material 101(mL/g),and extraction 2times,the yield of polysaccharide was(5.87±0.21)%(n=3).IR spectra predicted PTP had typical characteristic absorption peaks which were neutral polysaccharide and free from urinic acid.In vitro antioxidant activities showed PTP had effects on DPPH radical and hydroxyl radical.The IC_(50) of it against DPPH radical and hydroxyl radical was0.83mg/mL,1.29mg/mL.
【Fund】: 河北省中医药管理局项目(编号:2015082);; 河北中医学院博士启动基金(编号:BSZ2015005)
【CateGory Index】: TQ281
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