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Study on the extraction conditions of abalone harslet polysaccharide

CHENG Ting-ting,LI Dong-mei,LI Tao,ZHU Bei-wei(College of Biotechnology & Food Technology,Dalian Polytechnic University,Dalian 116034,China)  
The extraction technique of the polysaccharide from abalone harslet was studied.Six kinds of proteinase were applied to assist the polysaccharide extraction.The most suitable one was found to be the alkaline proteinase.Main factors during the extraction process were investigated.The results showed that the temperature and pH optima were 45℃ and 10.0,respectively.The optimal concentration of the substrate and alkaline proteinase were 2.5% and 2.0%,respectively.The optimum hydrolytic time was 3h.Under the above optimized conditions,the polysaccharide yield was 6.50%.
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