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《Science and Technology of Food Industry》 2008-11
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Research on esterase isoenzyme of Stropharia rugoso-annulate hypha in growth periods

ZHANG Qi-lin1,WANG Hong2(1.Department of Life Science,Yuncheng University,Yuncheng 044000 China;2.Centre of Biological and Chemical Experiment,Yuncheng 044000 China)  
Esterase isoenzyme analysis was one of researched indexes of edible mushroom idioplasm resource.The test results could be for reference in Stropharia rugoso-annulate physiological and biological-chemistry research and appliance.After Stropharia rugoso-annulate was cultivated 28d in synthetical PDA liquid culture medium,it was inoculated.The activity and kinds of Stropharia rugoso-annulate esterase were determined by polyacrylamide gel electrophoresis and α-Naphthyl acetate,β-Naphthyl acetate and Fast blue RR salt dyeing ways in the 7,10,13,16,19,22,25,28d.The result showed:five kinds of esterase isoenzyme were emerged,whose Rf were 0.48,0.56,0.65,0.73,0.87.Their emerging periods and activities were not same.They were all in existence from the 13~16d.All the time the enzyme stripe of Rf 0.56 was in enistence and its activity was stronger,which may be characteristic in hypha of Stropharia rugoso-annulate.
【Fund】: 山西省高校科技研究开发项目(200611043)
【CateGory Index】: S646.1
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