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《Science and Technology of Food Industry》 2012-10
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Effect of complex biological fresh-keeping agents for the main bacteria composition on cutlassfish Trichiurus haumela under the cold storage by PCR and physiology-biochemistry technology

LAN Wei-qing,XIE Jing(College of Food Science and Technology,Shanghai Ocean University,Shanghai Engineering Research Center of Aqhatic-product Processing & Preservation,Shanghai 201306,China)  
Microbial physiology-biochemistry technology in traditional method combined with PCR technology were used to study the effect of Complex Biological Fresh-keeping Agents(CBFA)on Trichiurus haumela.After the separation and purification of pre-dominate spoilage bacteria on Trichiurus haumela used by CBFA under the cold storage,16S rDNA sequence analysis,morphological and biochemical tests were used to identify the composition of spoilage microflora,and phylogenetic tree were analyzed.The result showed that there were mainly 13 typical bacterial strains of the controlled,and their proportions at the end of shelf life in control groups were:Shewanella putrefaciens 34.7%,Pseudomonas fluorescens 14.5%,Staphylococcus sciuri 10.2%,Aeromonas hydrophila 8.2%,Vibrio rumoiensis 6.1%,Pseudomonas putida 6.1%,Aerococcus viridans 4.1%,Staphyloccocus aureus 4.1%,Bacillus cereus 4.0%,Pseudomonas aeruginosa 2.0%,Psychrobacter sp.2.0% Enterobacter agglomerans 2.0% and Acinetobacter johnsonii 2.0% respectively.Among them,Shewanella putrefaciens(34.7%)was the Specific Spoilage Organism(SSO),the proportion of Pseudomonas sp.in descending order was Pseudomonas fluorescensPseudomonas putidaPseudomonas aeruginosa.It was found that the dominant spoilage bacteria of Trichiurus haumela was extended 9d by CBFA,the spoilage microflora was also changed,and the species of bacteria decreased to 9 strains.CBFA showed different inhibitory activity on different spoilage bacteria.
【Fund】: “十二五”国家支撑计划项目(2012BAD38B09);; 上海市科委工程中心建设(11DZ2280300);; 上海海洋大学博士启动基金(A-2400-11-0202);; 上海市教育委员会重点学科建设项目资助(J50704)
【CateGory Index】: TS254.7
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