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Effect of hydrochloric acid hydrolysis on antioxidant property of lotus leaf

JIANG Shen-hua,ZHOU Yong,XIAO Tong-fen,SHI Zhou-fang,CAI Zhi-peng,ZHANG Liang-hui,LIAO Liang*(College of Life Science,Jiujiang University,Jiujiang 332000,China)  
The effect of hydrochloric acid hydrolysis on antioxidant property of lotus leaf was studied based on that lotus leaf that was proved to possess strong antioxidant capacity.The result showed that the antioxidant capacities of lotus leaf were significantly improved after being hydrolyzed by hydrochloric acid.As for the crude extracts,the reducing power(OD700) was improved from 0.092±0.001 to 0.111±0.001,FRAP values was improved from 0.606±0.004 to 0.863±0.001,DPPH radicals scavenging percentage was improved from 29.371%±0.393% to 90.462%±0.393%,hydroxyl radical scavenging percentage was improved from 42.456%±2.391% to 73.196%±0.585%,superoxide anion radicals scavenging percentage was improved from 60.328%±1.808% to 77.330%±6.983%,respectively.As for the effective fraction,the total antioxidant capacity(OD695) was improved from 0.540±0.024 to 0.802±0.025.These results demonstrated that hydrochloric acid hydrolysis was a effective way to improve the antioxidant activity of lotus leaf.
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