Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Science and Technology of Food Industry》 2016-18
Add to Favorite Get Latest Update

Study on condition optimizing of extraction technique of squid ink polysaccharide from Sepia pharaonis by response surface methodology

JIANG Mao-wang;GAO Xiao-lan;HAN Qing-xi;PENG Rui-bing;JIANG Xia-min;School of Marine Sciences,Ningbo University;  
To explore the optimum extraction conditions of squid ink polysaccharides from Sepia pharaonis squid ink,this study was conducted to provide a theoretical basis for the development and utilization of squid ink. By single factor tests,enzyme dosage,solid- liquid ratio,reaction temperature,reaction duration and p H were investigated.And on the basis of single factor tests,according to the( Box- Behnken) experimental design protocol,enzyme dosage,solid- liquid- ratio,reaction temperature,and p H were selected to the four factors and the response surface experiment was carried out by response surface analysis. The results showed that their optimal conditions were defined as follows: enzyme amount 46 mg / g,solid- liquid- ratio 1∶ 19.7,reaction temperature 54.1 ℃and p H9.0. Under this condition,the polysaccharide yield was as high as 2.140%.
【Fund】: 国家自然科学基金项目(41206114);; 宁波市农业科技专项(2014C11001);; 宁波市科技重点项目(2011C11002)
【CateGory Index】: TQ281
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 10 Hits
1 GAO Xiaolan;JIANG Xiamin;LE Kexin;WANG Yuan;HU Xiaopeng;FENG Xun;College of Ocean,Ningbo University;;Analysis and Evaluation of Nutritional Components in Different Tissues of Wild Sepia pharaonis[J];动物营养学报;2014-12
2 GU Yi-peng;ZHANG Yun-bo;LIU Hua-zhong;LE Xiao-yan;TAO Ye-xing;XIAO Wei;LUO Ping;Faculty of Food Science & Technology,Guangdong Ocean University;Agricultural,Guangdong Ocean University;Biochemistry Center,Guangdong Ocean University;;Protective effects of squid ink polysaccharides against cyclophosphamide-induced ovarian damage in mice[J];食品工业科技;2014-17
3 GU Yi-peng;LUO Ping;School of Food Science & Technology,Guangdong Ocean University;;Interventional Effects of Sepia Polysaccharide against Cyclophosphamide-induced Oxidative Stress Injury to Testis of Mice[J];安徽农业科学;2014-19
4 SHI Xiao-li;YAO Chun-xia;LIN Xiao;SHEN Lan;FENG Yi;College of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine;Engineering Research Center of Modern Preparation Technology of Traditional Chinese Medicine of Ministry of Education,Shanghai University of Traditional Chinese Medicine;;The applications and research progresses of polysaccharide drugs[J];中国新药杂志;2014-09
5 CHEN Dao-Hai;ZHENG Ya-Long;Zhanjiang Normal University,Round Beibu Gulf Institute for the Protection and Utilization of Marine Animals in Medicine;Zhanjiang Normal University,College of Life Science and Technology;;THE REPRODUCTION ETHOGRAM OF CUTTLEFISH SEPIA PHARAONIS[J];海洋与湖沼;2013-04
6 CHEN Dao-Hai 1,WANG Yan 1,LIANG Han-Qing 1,SUN Guo-Xiang 2,WEN Jing 1,ZHENG Xiao-Dong 2(1.Zhanjiang Normal University,Life Science and Technology School,Zhanjiang,524048;2.Ocean University of China,Fisheries College,Qingdao,266003);STUDIES ON THE EMBRYONIC DEVELOPMENT AND THE HATCHING PERIODS OF SEPIA PHARAONIS[J];海洋与湖沼;2012-02
7 SUN Nan-nan,LI He-sheng,ZHANG Li-yuan,LEI Jing,ZHENG Xian-meng(College of Maritime Science,Ningbo University,Ningbo 315211,China);Extraction and Antioxidant Activity of Polysaccharides from Squid Ink[J];食品科学;2011-22
8 WU Jin-long1,LIU Hua-zhong2,SHI Li-sha1,WANG Guang1,HUANG Yan2,ZHONG Jie-ping3,PAN Jiang-qiu1, (1.College of Food Science & Technology,Guangdong Ocean University,Zhanjiang 524088,China;2.Modern Biochemistry Center,Guangdong Ocean University,Zhanjiang 524088,China;3.College of Science,Guangdong Ocean University,Zhanjiang 524088,China);Optimization of extraction of polysaccharide from cuttlefish ink[J];食品工业科技;2011-07
9 SHI Li-sha1,LIU Hua-zhong2,WU Jin-long1,CHEN Jiang-wei3,ZHANG Xu-ming3,ZHONG Jie-ping3*(1.College of Food Science & Technology,Guangdong Ocean University,Zhanjiang 524088;2.Modern Biochemistry Centre,Guangdong Ocean University,Zhanjiang 524088;3.College of Science,Guangdong Ocean University,Zhanjiang 524088);Extraction of polysaccharide in squid ink by enzymatic method[J];食品科技;2011-04
10 CHENG Ting-ting,LI Dong-mei,LI Tao,ZHU Bei-wei(College of Biotechnology & Food Technology,Dalian Polytechnic University,Dalian 116034,China);Study on the extraction conditions of abalone harslet polysaccharide[J];食品工业科技;2008-06
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 YANG Li-zhi;YANG Xian-qing;HUANG Hui;LI Lai-hao;DENG Jian-chao;ZHAO Yong-qiang;YANG Shao-ling;Key Laboratory of Aquatic Product Processing,Ministry of Agriculture,National R & D Center for Aquatic Product Processing,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Science;College of Food Science and Technology,Shanghai Ocean University;;Optimization of extraction technology polysaccharide from the ink of the Sthenoteuthis oualaniensis in South China Sea by response surface methodology[J];食品工业科技;2016-18
2 JIANG Mao-wang;JIANG Xia-min;GAO Xiao-lan;WANG Peng-shuai;RUAN Peng;HAN Qing-xi;School of Marine Sciences,Ningbo University;;Analysis and comparison of biochemical composition in squid ink between wild and cultured Sepia pharaonis[J];生物学杂志;2016-04
3 WANG Yan-hong;WANG Chao;SHANG Dong-xue;LI Yang;ZHAO Yong-wei;HAN Feng-juan;Department of Pharmacology,School of Pharmacy,Heilongjiang University of Chinese Medicine;First Gynecological Department,First Affiliated Hospital,Heilongjiang University of Chinese Medicine;;Determination of polysaccharide content and protein content in Lichong Shengsui Drink[J];哈尔滨商业大学学报(自然科学版);2016-04
4 ADELIJIANG Wusiman;DELIBAIER;NUERAILI Maitiniyazi;BUHELIQIANMU Yiming;SAIFUDING Abula;College of Veterinary Medicine,Xinjiang Agricultural University;;Study on Antiviral Effect of Polysaccharides from Lagenaria siceraria(Molina)Standl.on NDV in vitro[J];中国畜牧兽医;2016-07
5 ZHANG Zhe;LI Xin-pu;YANG Feng;LUO Jin-yin;LIU Long-hai;LI Hong-sheng;College of Veterinary Medicine,Gansu Agricultural University;Key Laboratory of New Animal Drug Project of Gansu Province,Key Laboratory of Veterinary Pharmaceutical Discovery,Ministry of Agriculture,Lanzhou Institute of Animal Husbandry and Pharmaceutical Sciences,Chinese Academy of Agricultural Sciences;;Study on Clinical Efficacy of Oligosaccharide Sulfate and Dextran Sulfate Against Bovine Mastitis[J];中国畜牧兽医;2016-07
6 SHI Hui-Lai;PING Hong-Ling;ZHANG Tao;Lü Zhen-Ming;MENG Hao;YU Fang-Ping;Zhejiang Province Key Laboratory of Marine Aquaculture, Marine Fisheries Research Institute of Zhejiang;National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University;;SEXUAL DIMORPHISM AND DISCRIMINATION OF CULTURED SEPIELLA JAPONICA[J];海洋与湖沼;2016-04
7 JING Yong-shuai;WU Lan-fang;WANG Qian;ZHENG Yu-guang;YAN Yu-ping;JIANG Ai-min;College of Chemistry and Pharmaceutical Engineering,Hebei University of Science and Technology;College of Pharmacology,Hebei University of Chinese Medicine;College of Food Science,South China Agricultural University;;Response surface methodology for optimization of extracting polysaccharide from Polygala tenuifolia willd.and antioxidant evaluation[J];食品与机械;2016-05
8 JIANG Maowang;JIANG Xiamin;LIANG Jingjing;WANG Pengshuai;RUAN Peng;HAN Qingxi;School of Marine Sciences,Ningbo University;;Comparative Analysis: Nutritional Component Contents in Cuttlebone of Sepia pharaonis at Different Growth Stages[J];动物营养学报;2016-07
9 WU Xuehao;HUANG Yuanxiang;XIE Xiujuan;WANG Ke;WAN Qiang;ZHANG Wei;HUANG Xiaowen;FAN Huajun;School of Pharmacy,Guangdong Pharmaceutical University;School of Basic Course,Guangdong Pharmaceutical University;;Determination of Monosaccharides in Polysaccharide Extracted from Sophorae tonkinensis Gagnep. by PMP Derivatization-High Performance Liquid Chromatography[J];今日药学;2016-05
10 JIANG Mao-wang;GAO Xiao-lan;HAN Qing-xi;PENG Rui-bing;JIANG Xia-min;School of Marine Sciences,Ningbo University;;Study on condition optimizing of extraction technique of squid ink polysaccharide from Sepia pharaonis by response surface methodology[J];食品工业科技;2016-18
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 CHEN Dao-hai;WEN Jing;ZHAO Yu-yan;LAI Jian-cai;Zhanjiang Normal University, Round Beibu Gulf Institute for the Protection and Utilization of Marine Animals in Medicine;Life Science and Technology School, Zhanjiang Normal University;;Analysis of Nutritional Components in Muscle of Cultivated and Wild Sepia pharaonis[J];食品科学;2014-07
2 LIU Hui-hui;CHI Chang-feng;LI Hai-feng;Zhejiang Ocean University, National Engineering Research Center of Marine Facilities Aquaculture;;Analysis of the Nutritional Composition of Pseudosciaena polyacti in Zhoushan[J];营养学报;2013-06
3 Lü Li, SUN Hui-jun, HAN Guo-zhu* (Department of Pharmacology, College of Pharmacy, Dalian Medical University, Dalian 116044, China);Research progresses of pharmacokinetics of traditional Chinese medicine[J];药学学报;2013-06
4 LE Xiao-yan;YUAN Lin;LIN Shao-jie;TAO Ye-xing;GU Yi-peng;LIU Hua-zhong;College of Food Science and Technology,Guangdong Ocean University;Biochemistry Center,Guangdong Ocean University;;Preparation of squid ink polysaccharides and its protection against chemotherapeutic injury on testis[J];食品工业科技;2013-17
5 LUO Ping1,SHI Li-sha1,LIU Hua-zhong2,*(1.College of Food Science & Technology,Guangdong Ocean University,Zhanjiang 524088,Guangdong,China;2.Modern Biochemistry Centre,Guangdong Ocean University,Zhanjiang 524088,Guangdong,China);Antioxidant Effects of Polysaccharides from Squid Ink in Vitro[J];食品研究与开发;2013-08
6 LIN Jun1,LI Ping1,CHEN Kao-shan1,2*(1.Department of Pharmacology,Wannan Medical College,Wuhu 241000,China; 2.School of Life Sciences,Shandong University,Ji′nan 250100,China);Advance in studies on anti-tumor activity of polysaccharides in latest five years[J];中国中药杂志;2013-08
7 GONG Qinqin1,ZHANG Changjun2,ZHANG Ying2,DONG Yifei2,RU Xue1,2,WANG Xiaoning1,2 1 The Research Center of Reproduction Medicine,The People's Hospital of Shiyan, Hubei University of Medicine,Hubei 442000,China 2 Hubei University of Traditional Chinese Medicine,Hubei 430065,China;Influence of cyclophosphamide on mouse sperm DNA and the function of curcumin interventio[J];中国性科学;2013-03
8 Qing-E Tian,School of Pharmaceutical Sciences,Central South University,Changsha 410011,Hunan Province,China Qing-E Tian,Xiangtan Central Hospital,Xiangtan 411100,Hunan Province,China Qing-E Tian,Huan-De Li,Miao Yan,Hua-Lin Cai,Qin-You Tan,Wen-Yuan Zhang,Clinical Pharmacy and Pharmacology Research Institute,the Second Xiangya Hospital,Central South University,Changsha 410011,Hunan Province,China;Astragalus polysaccharides can regulate cytokine and P-glycoprotein expression in H22 tumor-bearing mice[J];World Journal of Gastroenterology;2012-47
9 JIANG Xiamin PENG Ruibing LUO Jiang GAO Xiuzhi JIANG Xiaomin(College of Ocean,Ningbo University,Ningbo 315211,China);Analysis and Evaluation of Nutrient Composition in Different Tissues of Wild Sepia lycidas[J];动物营养学报;2012-12
10 LE Xiao-yan1,XU Ze-xuan1,SHI Li-sha1,LIN Shao-jie1,YUAN Lin1,LIU Hua-zhong2(1.Faculty of Food Science and Techology,Guangdong Ocean University,Zhanjiang 524088,China; 2.Biochemistry Center,Guangdong Ocean University,Zhanjiang 524088,China);Effects of sepia ink polysaccharides on spermatogenesis injured by cyclophosphamide[J];中国海洋药物;2012-05
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved