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《Science and Technology of Food Industry》 2016-18
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Study on condition optimizing of extraction technique of squid ink polysaccharide from Sepia pharaonis by response surface methodology

JIANG Mao-wang;GAO Xiao-lan;HAN Qing-xi;PENG Rui-bing;JIANG Xia-min;School of Marine Sciences,Ningbo University;  
To explore the optimum extraction conditions of squid ink polysaccharides from Sepia pharaonis squid ink,this study was conducted to provide a theoretical basis for the development and utilization of squid ink. By single factor tests,enzyme dosage,solid- liquid ratio,reaction temperature,reaction duration and p H were investigated.And on the basis of single factor tests,according to the( Box- Behnken) experimental design protocol,enzyme dosage,solid- liquid- ratio,reaction temperature,and p H were selected to the four factors and the response surface experiment was carried out by response surface analysis. The results showed that their optimal conditions were defined as follows: enzyme amount 46 mg / g,solid- liquid- ratio 1∶ 19.7,reaction temperature 54.1 ℃and p H9.0. Under this condition,the polysaccharide yield was as high as 2.140%.
【Fund】: 国家自然科学基金项目(41206114);; 宁波市农业科技专项(2014C11001);; 宁波市科技重点项目(2011C11002)
【CateGory Index】: TQ281
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