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《Food Science》 2005-07
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Study on Purification of Polyphenoloxidase in Flesh of Nanguo Pear by Ammonium Sulphate

XIN Guang1,ZHU Feng-mei2,LIU Chang-jiang2(1.Department of Chemistry, Anshan Normal University, Anshan 114005, China;2.Food Science College, Shenyang Agricultural University, Shenyang 110161, China)  
The purification of polyphenoloxidase(PPO) in flesh of Nanguo pear has been studied. At the end of the (NH4)2SO4precipitation and dialysis step, 2.1-fold purification was achieved. According to the gel electrophoresis analysis, two bands ofisoenzymes of crude polyphenoloxidase in flesh were expressed and Rf value respectively, 0.21, 0.26 and six bands ofpolyphenol oxidase in flesh after the (NH4)2SO4 precipitation were expressed and Rf value respectively, 0.21, 0.26, 0.30,0.39, 0.48 and 0.51.
【Fund】: 辽宁省教育厅高等学校科学研究资助项目(2024201055)
【CateGory Index】: TS255.1
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