Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Food Science》 2005-11
Add to Favorite Get Latest Update

Determination of Ginsenosides in Panax quinquefolium. L by HPLC and Study on Their Fingerprints Analysis

CHEN Jun-hui,XIE Ming-yong,WANG Hui-qin,PENG Ri-huang,WANG Yuan-xing(Key Laboratory of Food Science of Ministry of Education, Nanchang University, Nanchang 330047,China)  
A modern HPLC analysis method was developed for the determination of seven major ginsenosides in 12 kinds of Panax quinquefolium. L. Their chromatographic fingerprints were established by HPLC. Separations and determinations were carried out by linear gradient elution under following conditions: Alltima C18 (250mm×4.6mm, 5μm) column, acetonitrile-0.05% phosphoric acid solution as mobile phase, detection length of UV at 203nm, flow-rate of 1.2ml/min, column temperature of 35 ℃. The obtained results indicated that the developed method was simple, accurate and reliable for the determination of seven ginsenosides in Panax quinquefolium. L with a good linearity with the recovery ranges of 94.1%~97.9%. Hierarchical clustering analysis was performed and the similarity of the HPLC chromatograms was analyzed for the research of fingerprints and classificationo f Panax quinquefolium.L samples. Ther esultsc ouldp rovideu sefuli nformationf orq ualityc ontrolo fT raditional Chinese Medicine. The method was developed for the HPLC analysis and identification of complex components of samples.
【CateGory Index】: R284;
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 3 Hits
1 QIU Xiu-zhen(College of Chemistey & Environment Engineering,Shaoguan University,Shaoguan 512005 Guangdong,China);Fingerprint analysis of Ctrus grandis Var. Shatinyu Hout juice[J];Journal of Shaoguan University;2009-06
2 ZHOU Jing~1,DU Xiao-Wei~(2*),MA Hong-Yue~3,WU Jun-Kai~2(1.Pharmaceutical College,Nanjing University of Traditional Chinese Medicine,Nanjing 210046,China;(2.Pharmaceutical College,)Heilongjiang University of Chinese Medicine,Harbin 150040,China;3.Chinese Pharmacy College,China Pharmaceutical University,Nanjing 210038,China);Studies on the HPLC Fingerprints of Panax quinquefolium L.[J];LiShiZhen Medicine and Materia Medica Research;2006-12
3 YI Yan-kui1,DENG Hong-zhu1,LI Yue-hui2,YANG Yong-hua2 (1.Southern Medical University,Guangzhou,510515,China;2.Hunan Institute of TCM,Changsha,410006,China);Study on the Content Determination of Gingsenoside Re in Shuangshenlong Capsule by HPLC[J];Lishizhen Medicine and Materia Medica Research;2008-04
【Citations】
Chinese Journal Full-text Database 10 Hits
1 MA Xiao-Qiong, WANG Long-Xing, XU Qing, ZHANG Feng, XIAO Hong-Bin, LIANG Xin-Miao * (Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011, China);Optimization Method of Multi-segment Gradient for Separation of Ginsenosides in Reversed Phase-High Performance Liquid Chromatography[J];Chemical Research In Chinese Universities;2004-02
2 XU Chuan lian 1, ZHENG Yi nan 1, CUI Shu yu 2, HU Hong lai 2, LI Xiang 1 (1.College of Chinese Medicinal Material, Jilin Agricultural University, Changchun, Jilin 130118, China; 2 Xi′an Tiancheng Drug and Bio Engineering CO , X;Determination of Six Ginsenosides in Leaves and Stems of Panax quinuefolium L.by RP-HPLC[J];Journal of Jilin Agricultural University;2002-03
3 CHEN Wei, HU Guang lin, WANG Yi ru, WANG Xiao ru (The Key Laboratory of Modern Analytical Science of Ministry of Education and Department of Chemistry, Xiamen University, Xiamen 361005, China);Determination of Six Ginsenosides in Panax Species by High Performance Liquid Chromatography[J];Chinese Journal of Chromatography;2000-05
4 X Zhi xiu, XIAO Hong bin, WANG Jia ning,\ LIANG Xin miao (Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116012, China);Analysis of Ginsenosides by High Performance Liquid Chromato- graphy/Mass Spectrometry/Mass Spectrometry(LC/MS/MS)[J];Chinese Journal of Chromatography;2000-06
5 Qi Xiaoling; Lu Guibao; Guizhi Fenghe (Tianjin Municipal Institute for Drug Control. Tianjin 300070);The advance on the analysis methods of ginsenoside in panax ginseng CA Meyer, Panax quinquefolium Linn. and its preparations[J];TIANJIN PHARMACY;1998-02
6 ZHOU Jing , QIAO Wei , FU Jingwei , FENG Shuhua,LU Zhijin,CHEN Qimeng ( Department of Pharmacy , Tianjin Medical University,300203);Effect of different extracting methods on quinquefolium sapanins[J];JOURNAL OF TIANJIN MEDICAL UNIVERSITY;2000-01
7 Han Yixin;Xu Xiuzhen(Ningxia Institute for Drug Control);COMPARISON OF TWO EXTRACTION METHODS IN THE QUINQUENOMDES CONTENT DETECTION OF PANAX QUINQUEFOLIUM[J];NINGXIA MEDICAL JOURNAL;1996-02
8 MIAO Ai dong, SUN Dian jia(The PLA 474 Hospital, Urumqi 830011, China);Application of Microsoft Excel 2002 to Calculate the Similarity in Fingerprints of Chinese Herbs[J];Progress In Pharmaceutical Sciences;2003-01
9 JIA Xiao-bin 1, SHI Ya-fang 1, CHEN Yan 1, HUANG Yi-ping 1, WAN Xiao-lei 1, FAN Gan-bin 2 (1. Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China; 2. Nanjing Donfang Pharmacentical Factory, Nanjing 210062, China);Study on HPLC fingerprint of Compound Renshen Injection[J];Chinese Traditional Patent Medicine;2002-04
10 Wen Liyu;Yu Yang;Li Binggen (Yangzhou Institute for Drug Control,Yangzhou 225001) Xu Boyi;Chen Zhiqing;Hu Yue (Jiangsu Suzhong Pharmaceutical Factory);Determination of Ginsenoside Re in Shengmai Injection by HPLC[J];CHINESE TRADITIONAL AND HERBAL DRUGS;1998-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Li-yuan, CHENG Hao*, ZHOU Jian, YE Yang, LIU Xu, LU Wen-yuan (Key lab of tea chemical engineering of Agricultural Ministry China, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China);A Preliminary Attempt to Establish the Digital Multiple Chemical Fingerprints of Green Tea[J];Journal of Tea Science;2007-04
2 ZHUANG Jiang-neng1,ZHANG Hai2,LIU Xiao-fan3,ZHAO Liang2,ZHU Zhen-yu3,ZHANG Guo-qing2,CHAI Yi-feng31.Department of Medical Adminstration,No.62 Hospital of PLA,Puer 665000,China2.Department of Pharmacy,Eastern Hepatobiliary Hospital,Second Military Medical University,Shanghai 2004383.Department of Pharmaceutical Analysis,School of Pharmacy,Second Military Medical University,Shanghai 200433;HPLC-TOF/MS in identification of chemical components in Ginseng[J];Academic Journal of Second Military Medical University;2009-07
3 LI Xue-long,YANG Bo,XU Peng,FU Shao-ping,YU Hong-shan,JIN Feng-xie Fermentation Engineering Key Laboratory of Liaoning Province,Dalian Polytechnic University,Dalian 116034,China;Establishment of high performance liquid chromatographic fingerprint of Fructus schisandrae sphenantherae[J];Journal of Dalian Polytechnic University;2009-01
4 DU Zeng-kai,CHENG Zhou,YANG Xiao-ling,WAN Shu-wen,ZHANG Wen-ju (School of Life Science and Technology ,Tongji University, Shanghai 200092,China;Shanghai Lei-yun-shang Medicine Co. Ltd. , Shanghai 200002,China;Institute of Biodiversity Science ,School of Life Science ,Fudan University, Chinese Ministry of Education Key Laboratory of Biodiversity Science and Ecological Engineering,Shanghai 200433,China);Comparative Anatomical Study of the Roots of Transplanted Panax ginseng Plants Growing for Different Times[J];Acta Botanica Boreali-Occidentalia Sinica;2006-04
5 Liao Huajun(Department of Pharmacy,Fujian college of Traditional Chinese Medicine,Fuzhou 350108);Development of Extraction New Technology in Traditional Chinese Medicine[J];Fujian Analysis & Testing;2008-01
6 LI Lu1,CHENG Wei1,YANG Jie2(1.Hubei Academy of Agricultural Sciences Institute for Farm Products processing and Nuclear Agricultural Technology,Wuhan 430064,China;2.Food Quality Supervision Inspection and Testing Center(Wuhan)of Chinese Ministry of Agriculture,Wuhan 430064,China);Determination of Ginsenosides in Panax Japonicus C.A.Mey by HPLC[J];Analysis and Testing Technology and Instruments;2008-01
7 Cao Jin, Xu Yan, Wang Yiming, Luo Guoan* (Analysis Center of Chemistry Department, Tsinghua University,Beijing 100084);Determination of Macro-porous Resin Residue in Herba Hyperici Perforat Extraction by Flow Gas Capture Method[J];Chinese Journal of Analytical Chemistry;2004-03
8 CHENG Hong-ying1,2,FENG Qi-yu1,CAO Yu-hua*1,GONG Li-dong1,HOU Jian-xia1(1.School of Chemistry and Material Engineering,Southern Yangze University,Wuxi,Jiangsu 214122;2.Department of Chemistry and Chemical Engineering,Suzhou Scienceand Technology College,Suzhou,Jiangsu 215009);Determination of Active Ingredients in Chrysanthemum and Development of Fingerprints by High Performance Liquid Chromatography[J];Journal of Analytical Science;2007-03
9 CHAI Yi-feng, WU Yu-tian and LI Xiang(Department of Pharmaceutical Analysis, College of Pharmacy, Second Military Medical University, Guohe Road 325, Shanghai 200433), Fenxi Shiyanshi,2004,23(6):71~92;Pharmaceutical Analysis I[J];Analytical Laboratory;2004-06
10 Wang Chunhao~1 Wang Yanji~1 Meng Pinjia~1 Yang Ruiqin~1 Wu Dehua~2 (1. Chinese People's Public Security University,Beijing ,100038; 2. Nanjing Police Dog Research Institute of Public Security Ministry,Nanjing,210012);Study on conservation method for human odour.[J];Analytical Instrumentation;2008-02
China Proceedings of conference Full-text Database 5 Hits
1 CAI Ze-fan ZHU Xue-feng LAI He-ji ZHANG Feng(College of Automation Science & Engineering,South China University of Technology,Guangzhou 510640);Correction of the baseline shift in HPLC based on wavelet transform[A];[C];2005
2 ZHOU Wenbing, Cai Yongming and Chen Huayan (GuangDong Pharmaceutical University, Medical Information Engineering Institute, Guangzhou, 510006);Characteristic peakes integral of the chromatographic fingerprint of the Chinese native medicine based on merged fingerprint[A];[C];2006
3 JIANG Hai-ping~1 DOU De-qiang~1 JING Shu-qin~2 LIU Feng-yun~2 1.College of Pharmacy,Liaoning University of Traditional Chinese Medicine,77 Life One Road DD port,Dalian 116620; 2.Benxi Institute For Drug Control;Study on ginsenoside and fingerprint of linxiashanshen by HPLC[A];[C];2007
4 YU Jian-sheng(Tongren Vocational Institute,Tongren 554300,china);Separation and purification of flavonoids from Climbing Groundsel Herb with macroporous resin[A];[C];2008
5 Cheng Hao, Wang Li-yuan, Zhou Jian, Ye Yang, Liu Xu, Lu Wenyuan (Key lab of tea chemical engineering of Agricultural Ministry China, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, 310008);Preliminary Studies on the Digital Multiple Chemical Fingerprints of Green Teas[A];[C];2007
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Cheng Shiming Zhang Shuhai Zhang Jinglin(North China Engineering College,Taiyuan 030051);Application in Research of Supercritical Fluid Technology[J];Anhui Chemical Industry;2003-01
2 Yong Ji Pan Jian Zhang Wencheng (The Key Laboratory of Agricultural Product & Biochemistry of the Ministry of Education, Hefei University of Technology, Hefei 230069, China);Study on Supercritical Fluid Extraction of Flavonoids[J];Anhui Chemical Industry;2005-03
3 MA Yan-fang(Department of Chemistry,Qinghai University for Nationalities,Xining,Qinghai 810007);Study on Antioxidative Effect of Lonicera Similis Hemsl[J];Journal of Anhui Agricultural Sciences;2007-11
4 Wang Qingping;Zhou Jiaqi;Wang Lingxian(Biotechnology Research Institute of Yunnan Academy of Agricultural Sciences 650223);Nutrition of the Amino Aicids in Yunnan Lijiang Panax ginseng and Panax quinquefolium[J];;1994-04
5 Li Jing;Wei Yongdi(Bethune University of Medical Science, changchun 130021);Amino Acid Contents in the Leaves of Wild, Cultivated Ginseng and Panax quinquefolium[J];AMINO ACIDS & BIOTIC RESOURCES;1996-02
6 HUANG Shu-ming~(1,4),YANG Xin-lin~1,HUANG Jian~1,XU Jian-lan~2,ZHU He-sun~3 (1.School of Life Science and Technology, Beijing Institute of Technology, Beijing100081, China; 2.Sanlian Hi-Tech Limited Company, Wuxi, Jiangsu214023, China; 3.School of Material Science and Engineering, Beijing Institute of Technology, Beijing100081, China; 4.Bengbu Technological Academy, Bengbu, Anhui233030, China);Method for the Rapid Quantification of Triterpenoids in Ganoderma Lucidum by Ultraviolet Spectrometry[J];Journal of Beijing Institute of Technology;2004-06
7 LI Zhi-zhou(Dept.Chem.,Shaanxi Univ.Tech.,Hanzhong 723001,Shaanxi,China);Extraction method and anti-oxidation property of flavones from Lonicera Japonica Thunb's floral axises[J];Journal of Baoji University of Arts and Sciences(Natural Science Edition);2006-02
8 Zhang Jiasheng, Ye Hanguang, An Ruguo, Bu Fengquan, Shi Yi, Li Wenxing, Liu Zhongying and Zhang Guitian;Determination of trace elements in different parts of Panax quinquefolius Linn in China[J];;1987-06
9 Li Xinmin, Liu Shuzheng, Ma Xingyuan and Xu Jingda (Laboratory of Endocrinology, Department of Chemistry);A study of the effect of ginsenosides on the secretion of gonadotropins[J];;1988-04
10 Lu Zhongzhi, Zhang Qinglan, Yu Xiaofeng, Ma Xingyuan(Dept.of Pharmacol., Dept.of Org.Chem);PROTECTIVE EFFECT OF PQS ON RABBITS WITH ACUTE MYOCARDIAL INFARCTION[J];;1990-03
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 ZHANG Da-li,YANG Xiao-fang,FENG Shu-hua (Biochemical Engineering College of Beijing Union University,Beijing,100023,China);Researching HPLC Fingerprint of Radix Ophiopogonis Decoction by direct-vision Comparison Method[J];Lishizhen Medicine and Materia Medica Research;2008-03
2 LI Tao1,2,QU Xin-yun3,WANG Zhe-zhi1,2(1.Key Laboratory of Ministry of Education for Medicinal Plant Resource and Natural Pharmaceutical Chemistry;2.College of Life Sciences;3.Science and Engineering College,Shanxi Normal University,Xi′an 710062,China);Study on RP-HPLC Fingerprints of Panax quinquefolium from Liuba County in Shanxi Province[J];Journal of Chinese Medicinal Materials;2008-08
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Xu Yongqun 1,3 , Sun Suqin *1 , Yuan Zimin 2, Bai Yan 2 1(Department of Chemistry, Tsinghua University,Beijing 100084) 2(Henan College of Traditional Chinese Medicine,Zhengzhou 450008) 3(Department of Chemistry, Huanggang;Discrimination of Trueborn Tuber Dioscoreae by Fingerprint Infrared Spectra and Principal Component Analysis[J];Chinese Journal of Analytieal Chemistry;2002-10
2 Shan Yichu, Zhao Ruihuan, Zhang Weibing, Liang Zhen, Zhang Yukui * (Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011);Fast Optimization Method of Binary Multi-segment Gradient Elution Conditions for the Separation of Complex Samples in Reversed Phase-High Performance Liquid Chromatography[J];Chinese Journal of Analytieal Chemistry;2002-12
3 Xu Yongqun 1,3 , Huang Hao 1, Zhou Qun 1, Zhou Hongtao 2, Hu Shilin\+2, Sun Suqin *1 1(Department of Chemistry, Tsinghua University,Beijing 100084) 2(Chinese Academy of Traditional Chinese Medicine, Beijing 100700) 3(Department of Chemistry, Huanggang Teachers College, Huanggang 438000;The Application of Fingerprint Infrared Spectra and Clustering Analysis in the Discrimination of Geographical Origin of Paeonia lactiflora Pall[J];Chinese Journal of Analytical Chemistry;2003-01
4 XU Yong-qun 1,3, SUN Su-qin 1*, FENG Xue-feng2 and HU Shi-lin2 1. Department of Chemistry, Tsinghua University, Beijing 100084, China 2. Chinese Academy of Traditional Chinese Medicine, Beijing 100700, China 3. Department of Chemistry, Huanggang Normal University, Huangguang 438000, China;Quick Identification of Scullcaps in Different Geographical Origins Using Clustering Analysis Method and Infrared Fingerprint Spectra[J];Spectroscopy and Spectral Analysis;2003-03
5 ZHOU Yu SONG Feng-Rui LIU Shu- Ying (Changchun Institute of Applied Chemistry, The Chinese Academy of Scienses, Changchun, 130022) LI Xiang - Gao (Jilin Argriculture University, Insititute of Traditional Chinese Medicine, Changchun, 130118 );Matrix - Assisted Laser Desorption/Ionization of Ginsenosides[J];Acta Chimica Sinica;1998-03
6 Zhang Chongxi;STUDY ON THE CHEMICAL COMPOSITION OF AMERICAN GINSENG (PANAX QUINQUEFOLIUM)GROWN IN CHINA[J];Journal of Jilin Agricultural University;1988-01
7 Qin Hailin (Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing100050,China);Fine expression and analysis of total chemical characteristics of material foundation of Traditional Chinese Herbal Medicines[J];;2002-04
8 CHEN Wei, HU Guang lin, WANG Yi ru, WANG Xiao ru (The Key Laboratory of Modern Analytical Science of Ministry of Education and Department of Chemistry, Xiamen University, Xiamen 361005, China);Determination of Six Ginsenosides in Panax Species by High Performance Liquid Chromatography[J];Chinese Journal of Chromatography;2000-05
9 SHI Xian zhe, YANG Jun, ZHAO Chun xia, XIONG Jian hui, XU Guo wang (National Chromatographic R.&A. Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116011, China);Application of Fingerprint Chromatogram in Quality Control of Shen-Mai Injection[J];Chinese Journal of Chromatography;2002-04
10 Gu Guanghua;Zhou Lei and Zhao Guiwen(University of Scicnce & Technology of China,Hefei,230026);Improved High Performance Liquid Chromatographic(HPLC)Methodfor the Analysis of Ginsenosides in Panax Ginseng Extracts & Products the Chromatographic Modifier Action of Ammonium Acetate in Separation of Ginsenosides[J];CHINESE JOURNAL OF CHROMATOGRAPHY;1994-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved