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Isolation and Identification of An Endophytic Strain EJS-3 Producing Exopolysaccharide

SUN Li-jun1,2,LU Zhao-xin1,*,LIU Jun1,LU Feng-xia1,BIE Xiao-mei1,YANG Sheng-yuan1 (1.College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; 2.School of Industry, Anhui Science and Technology College, Fengyang 233100, China)  
An endophytic Strain EJS-3 producing exopolysaccharide (EPS) of 23.6g/L was derived from tissues of Stemona japonica (Blume) Miq and identified on the base of 16S rRNA gene sequences. The sequence fragment of 1450bp 16S ribosomal RNA was amplified from total DNA of strain EJS-3 by PCR. The sequence analysis was complete by the application of BLAST on the websites of NCBI and phylogenetic tree was drawn with Bioedit 7.0 and Treedrawing. The results showed that EJS-3 shares 99.31% 16S rRNA sequence homology with Paenibacillus polymyxa KCTC 1663. In phylogenetic framework of bacteria classification, strain EJS-3 belongs to Paenibacillus polymyxa.
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