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《Food Science》 2006-07
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Optimal Design of Extraction and Separation Procedure for the Usnea subfloridana Polysaccharide

LIU Li-Yan,WANG Li,WEN Xue-Mei,Abdulla.ABBAS* (College of Life Science and Technology, Xinjiang University, Urumqi 830046, China)  
This paper studied the optimum preparation procedure for extraction and septeration of the Usnea subfloridana polysaccharide. The optimum extraction conditions of Subfloridana polysaccharide are: water 200ml, Usnea subfloridana 4g, extracting-temperature 70~80℃, repeated-times 3 and extracting-time 2h. The results showed that the variance of the factor reapeated-times assumes statistical significance, whereas the extracting-temperature is not statistically significant. A one-factor test results indicated that the optimum conditions of precipitating polysaccharide with 70% alcohol, and removing proteins with sevage agent to gain gratifying effictiveness are as follows: volume ratio 2:1, oscillation-time 30min and repeated times 7 with polysaccharide yield 6.86% and total sugar content 76%.
【Fund】: 国家自然科学基金资助项目(30460001;30160001);; 新疆生物资源基因工程重点实验室资助项目(040080)
【CateGory Index】: TQ28
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