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《Food Science》 2006-11
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Simultaneous Determination of the Four Lignans in Extract of Schisandra chinensis and S.sphenanthera by TLCS

YANG Liu-qing1,XU Zuo-qi1,WU Xiang-yang2,QI Xue-yong1,FAN Qun-yan3 (1.School of Pharmacy,Jiangsu University,Zhenjiang 212013,China; 2. School of Chemistry and Chemical Engineering,Jiangsu University,Zhenjiang 212013,China; 3.School of Food and Biological Engineering,Jiangsu University,Zhenjiang 212013,China)  
Objective: To establish a method for determining the content of the four lignans in extract of Schisandra chinensis and S.sphenanthera simultaneously. MethodSamples were extracted with alcohol and CO2-SFE. The contents of schisandrin,Wuweizi ester A,schisandrin A and schisandrin B could be determined simultaneously by dual wavelength TLC- Scanning (λS=220nm,λR=340nm) after the extracts were developed by n-hexane- ethyl acetate (8:3) on self-made GF254 plates. ResultThe calibration curves were linear in range of 0.44~1.54μg for schisandrin(R=0.9986),0.60~2.10μg for Wuweizi ester A(R=0.9960),0.52~1.82μg for schisandrin A(R=0.9997) and 0.48 ~1.68μg for schisandrin B(R=0.9962) respectively. The average recoveries(n=5) were 104.5% for schisandrin(RSD=4.0%),95.0% for wuweizi ester A(RSD=2.8%),96.1% for schisandrin A (RSD=2.6%) and 102.1% for schisandrin B(RSD=1.9%). All of the contents of four lignans in extracts of schisandra with SCF- CO2 are higher than that in extracts of schisandra with alcohol. Conclusion: This method was found to be simple,fast and accurate for simultaneously determining the content of the four lignans in extract of Schisandra chinensisl and S. sphenanthera.
【Fund】: 江苏省高校自然科学研究基金资助项目(02KJD350001)
【CateGory Index】: R284.1
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