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Determination of Total Polyphenolic Compounds in Bee-pollen by Folin-Ciocaileu Method

TIAN Wen-li,SUN Li-ping,DQNG Jie,LIU Bo (Bee Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100093, China)  
There are rich polyphenolic compounds in bee-pollen, which have multi-functions, such as anti-arteriosclerosis, cholesterol lowering function, anti-oxidation and anti-radiation. Therefore, polyphenolic compounds could be evaluated as the activities of bee-pollen. Total polyphenolic compounds in bee-pollen were determined by Folin-Ciocaileu method. The standard sample is protocatechuic acid, and regressive equation of standard curve is C=7.9234A-0.0157 (R2=0.9999). Furthermore, stability, precision, repetition and recovery rate were assayed with RSD(relative standard deviation) as 0.05%~1.43%. The method is convenient, fast, and exact, it is reliable to determine the total polyphenolic in bee-pollen.
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