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《Food Science》 2007-09
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Study on Technology of Extraction and Purification Superoxide Dismutase from Yellow Mealworm

WU Lei1,CHEN Qing-sen1,LIU Jin-sheng2(1.Tianjin Key Laboratory of Food and Biotechnology,Department of Bioengineering,Tianjin University of Commerce,Tianjin 300134,China;2.School of Chemical-Engineering,Tianjin University,Tianjin 300072,China)  
In this paper,a kind of superoxide dismutase(SOD)was obtained from yellow worm powder.Two-step(NH4)2SO4 precipitation were used as the main extraction method and DEAE-Sepharose FF ion-exchange and Sephadex G200 volume exclude chromatography were used as purification methods.The purification factor was 78.3.The SDS-PAGE showed that the SOD was a homogenous protein.This enzyme was proved as Cu.Zn-SOD.This study provides a suitable technology for obtaining SOD from the yellow mealworm.
【Fund】: 天津市科技攻关计划重点专项项目(06YFGZNC04200)
【CateGory Index】: Q814.1
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