Antitumor Activity of Extracellular Polysaccharides from Morchella esculenta
CHEN Yan1,2,PAN Jian1,ZHOU Li-wei3,ZHANG Li-ping2,YANG Yi1 (1.Engineering Research Center of Bio-process,Ministry of Education,Hefei University of Technology,Hefei 230009,China; 2.School of Life Science,Anhui University,Key Laboratory of Chinese Traditional Medicine Research and Development of Anhui Province,Hefei 230039,China;3.College of Tea and Food Science Technology,Anhui Agricultural University,He fei 230036,China)
Objective: To study the antitumor effects of extracellular polysaccharides from Morchella esculenta (EPM) and the immunological function on S180 tumor-bearing mice. Method: EPM was intragastrically given to S180-bearing mice at the dose of 200 mg/kg and 400 mg/kg. Normal sodium and cyclophosphamide were taken as blank control and positive control respectively. The tumor weight, immunologic organ weights and life span of S180-bearing mice were measured. S180 tumor cells were incubated with various concentrations of EPM for 24 h, and antiproliferative activities against tumor cells were determined by MTT assay. Result: EPM significantly increases the spleen index, percent of T-lymphocyte, macrophage phagocytic index and prolongs survival time of S180-bearing mice.The inhibiting tumor rates at the low and high does are 49.04% and 64.42% respectively. EPM can kill S180 tumor cells directly in vitro, with the highest inhibiting rate 46.3% at the dose of 50μg/well.Conclusion: EPM has significant antitumor effects and improves immunological function.