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《Food Science》 2009-03
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Optimization of Fermentation Medium Composition for Production of Extracellular Glucose Oxidase by Aspergillus niger Z-25

ZHANG Ting,L Feng-xia,BIE Xiao-mei,ZHAO Hai-zhen,WANG Yu,LU Zhao-xin(College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China)  
Effects of glucose addition amount,organic nitrogen source kind,proteose peptone addition amount,inorganic nitrogen source kind,(NH4)2SO4 addition amount,Tween type and Tween 80 addition amount in medium on production of extracellular glucose oxidase by Aspergillus niger Z-25 fermentation were studied in single factor tests,respectively.Then the addition amounts of glucose,(NH4)2SO4,proteose peptone and Tween 80 were optimized through orthogonal test.Results showed that the optimal addition amounts are as follows:glucose 10%,(NH4)2SO4 0.5%,Tween 80 3% and proteose peptone 1.5%.By optimization,the activity of produced glucose oxidase is increased by 1.42 times.
【CateGory Index】: TS201.3
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