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《Food Science》 2009-08
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Application of Response Surface Methodology to Optimization of Polysaccharide Extraction Technology from Coprinus comatus

YU Jie,CUI Peng-ju,ZHUO Jian-yong,CHEN Mei-zhen(Department of Biology,College of Science,Shantou University,Shantou 515063,China)  
Effects of extraction temperature,extraction time,ratio of water to material and extraction times on extraction rate of polysaccharide from Coprinus comatu were investigated respectively in single-factor tests,and then other three key factors except extraction times were optimized through response surface methodology with three factors and three levels based on the principle of Box-Behnken design.The results showed that the optimum extraction temperature,time,ratio of water to material and times are 94.5 ℃,.4 h,15.5:1 and 2,respectively.Under the above conditions,the extraction rate of polysaccharide from Coprinus comatu reaches 6.16%,which is improved by 7.13% through optimization.
【CateGory Index】: S646.19
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