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《Food Science》 2009-12
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HPGPC Determination of Relative Molecular Mass of Inulin

ZHANG Ze-sheng,ZHANG Jian-ping,SHI Shen,SHI Jia-ning,ZHANG Jie(Laboratory of Food Additives and Nutrition,Tianjin University of Science and Technology,Tianjin 300457,China)  
A high performance gel permeation chromatography(HPGPC) method was developed for the determination of relative molecular mass of inulin.Separation was performed on a GPC column with triple distilled water as a mobile phase at the flow rate of 0.8 ml/min and 30℃ and determination was carried out using a refractive index detector.A direct linear relationship within the range of 1.0×103-2.5×105 of relative molecular mass(r = 0.9894) was found between retention time of dextranum and lgMw.Insulins obtained from jerusalem artichoke by hot water reflux method and ultrasonic-assisted and microwave-assisted water extraction methods possessed varying relative molecular mass of 3500,2405 and 3864,respectively.Methodology evaluation experiments revealed that the RSDs for precision,repeatability and stability were 0.24%,0.21%,and 0.38%,respecitively,and the relative error for accuracy was around 0.4%.This method is proved to be fast,simple,sensible and repeatable.
【CateGory Index】: TS207.3
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