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《Food Science》 2010-06
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Dilution-PCR and PCR-DGGE for Evolution of Effect of Pre-treatment Methods on Extraction of Bacterial DNA from Chilled Pork

JIANG Yun1,2,GAO Feng1,XU Xing-lian1,YE Ke-ping1,ZHOU Guang-hong1,*(1.National Center of Meat Quality and Safety Control,Nanjing Agricultural University,Nanjing 210095,China;2.Ginling College,Nanjing Normal University,Nanjing 210097,China)  
Dilution-PCR and PCR-DGGE were used to evaluate effect of four pre-treatment methods such as freeze-thaw,ultrasonification,shake and homogenization on extraction of bacterial DNA from chilled pork in this study.After 4 days of storage at 4 ℃,chilled pork was subjected to pre-treatment by the above methods for bacterial DNA extraction followed by dilution-PCR and PCR-DGGE analyses.Results showed that four pretreatment methods results in a slight difference in bacterial DAN yield but had no notable effect on PCR-DGGE analysis.Therefore,these pretreatment methods could be chosen according to actual requirement.Sequence analysis of DGGE bands of 16S rRNA gene V3 region showed that the predominant spoilage bacterium in chilled pork was Pseudomonas and others including Acinetobacter,Brochothrix,and Serratia were also detected.
【Fund】: 国家“863”计划重点项目(2008AA100804);; 江苏省高校自然科学基础研究项目(08KJB550005);; 上海市科委重大攻关项目(07dz19508)
【CateGory Index】: TS251.51
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【Co-citations】
Chinese Journal Full-text Database 1 Hits
1 WANG Meng-zhi,LI Guo-xiang,WANG Hong-rong*,ZHANG Jie,CAO Heng-chun (College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China);Effects of Different Dietary Cellulose Levels on Microbial Recovery Rate after Detaching and DNA Extraction Rate in vitro[J];Chinese Journal of Animal and Veterinary Sciences;2008-02
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 WU Duogui,LIN Qifeng,LI Guanyi (Institute of Biological Science and Technology,Hainan University,Haikou 570228);Cetyl trimethyl Ammonium Bromide(CTAB)Method for Extracting Rhizophora apiculata's DNA and its Random Amplified Polymorphic DNA(RAPD)Reaction[J];CHINESE JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY;1999-01
2 Zhu Weiyun * Yao Wen Mao Shengyong (College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China);Development of Bacterial Community in Faeces of Weaning Piglets as Revealed by Denaturing Gradient Gel Electrophoresis[J];Acta Microbiologica Sinica;2003-04
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