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《Food Science》 2011-01
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Suppressive Effect of Rhizoma sparganii Flavonoids on HeLa Cells (I):Induce M Stage Arrest and Cell Apoptosis

SUN Jie,WANG Shao,GUO Bin,WEI Ya-hui (Key Laboratory of Resource Biology and Biotechnology in Western China,Ministry of Education,College of Life Science, Northwest University,Xi'an 710069,China)  
Objective:To explore the toxicity effect and mechanisms of Rhizoma sparganii flavonoids(RSF) on HeLa cells.Methods:The effect of RSF at a concentration range of 11.7 to 750μg/mL on the proliferation of HeLa cells was evaluated.The toxic mechanisms were elucidated by immunofluorescence,flow cytometry and karyotype analysis.Results:RSF at a concentration higher than 188μg/mL could significantly(P0.01) inhibit the proliferation of HeLa cells in a dose-dependent manner,and increase the proportion of apoptotic bodies(P0.01).Compared with the control group,the RSF treatment groups at doses of 375μg/mL(RSF-375) and 750μg/mL(RSF-750) revealed a significant decrease of HeLa cells number at M stage(P0.01),disappearance of intracellular interaction and an increase of cell volume.In addition,RSF-375 and RSF-750 also showed irregular morphological changes,an increase of synaptics and higher moving trend of microfilament,which were observed in monitoring the specific immnuofluorescence of microtubule protein.Conclusion:RSF is a group of bioactive components against HeLa cervical cancer cells,with the physiological functions of significantly inhibiting proliferation and inducing apoptosis by interfering the mitosis of HeLa cells.
【Key Words】： Rhizoma sparganii flavonoids HeLa cell cervical cancer
【Fund】： 国家自然科学基金项目(31000144);; 陕西省教育厅专项(09JK746);; “十一五”国家科技支撑计划项目(2006BAD06B00)
【CateGory Index】： R285.5
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