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《Food Science》 2011-01
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Suppressive Effect of Rhizoma sparganii Flavonoids on HeLa Cells (I):Induce M Stage Arrest and Cell Apoptosis

SUN Jie,WANG Shao,GUO Bin,WEI Ya-hui (Key Laboratory of Resource Biology and Biotechnology in Western China,Ministry of Education,College of Life Science, Northwest University,Xi'an 710069,China)  
Objective:To explore the toxicity effect and mechanisms of Rhizoma sparganii flavonoids(RSF) on HeLa cells.Methods:The effect of RSF at a concentration range of 11.7 to 750μg/mL on the proliferation of HeLa cells was evaluated.The toxic mechanisms were elucidated by immunofluorescence,flow cytometry and karyotype analysis.Results:RSF at a concentration higher than 188μg/mL could significantly(P0.01) inhibit the proliferation of HeLa cells in a dose-dependent manner,and increase the proportion of apoptotic bodies(P0.01).Compared with the control group,the RSF treatment groups at doses of 375μg/mL(RSF-375) and 750μg/mL(RSF-750) revealed a significant decrease of HeLa cells number at M stage(P0.01),disappearance of intracellular interaction and an increase of cell volume.In addition,RSF-375 and RSF-750 also showed irregular morphological changes,an increase of synaptics and higher moving trend of microfilament,which were observed in monitoring the specific immnuofluorescence of microtubule protein.Conclusion:RSF is a group of bioactive components against HeLa cervical cancer cells,with the physiological functions of significantly inhibiting proliferation and inducing apoptosis by interfering the mitosis of HeLa cells.
【Fund】: 国家自然科学基金项目(31000144);; 陕西省教育厅专项(09JK746);; “十一五”国家科技支撑计划项目(2006BAD06B00)
【CateGory Index】: R285.5
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