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《Food Science》 2011-22
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Extraction of Polysaccharides from Eclipata Alba Grown in Huaguo Mountain and Comparison of Antioxidant Activity in vitro with Total Flavonoids from Eclipata Alba

XU Rui-bo1,2,WANG Xin-xin2,TANG Qiu-ping2,HU Xi-lan2,ZHAN Yong-cheng2 (1.Jiangsu Key Laboratory of Marine Biotechnology,Lianyungang 222005,China; 2.School of Chemical Engineering,Huaihai Institute of Technology,Lianyungang 222005,China)  
Using one-factor-at-a-time experiments followed by orthogonal array design,the process conditions of polysaccharide extraction from Eclipata Alba grown in Huaguo Mountain were optimized in this study.Also,a comparison of antioxidant activity was carried out between polysaccharides(PEA) and flavonoids(FEA) from Eclipata Alba.The results showed that the optimum conditions for polysaccharide extraction were material-to-liquid ratio of 1:15(g/mL),extraction time of 1 h,extraction temperature of 90 ℃ and extraction number of 3.After the removal of impurities such as proteins,nucleic acids,etc,the extracted polysaccharides were characterized using ultraviolet spectroscopy and infrared spectroscopy.Both PEA and FEA had a powerful ability to scavenge hydroxyl and superoxide anion radicals and could scavenge the former more powerfully.At the same concentrations exceeding 0.085 mg/mL,FEA indicated stronger antioxidant activity than PEA.
【Fund】: 江苏省海洋生物技术重点建设实验室开放课题(2009HS06);; 江苏省青蓝工程优秀青年骨干教师资助项目(2008);; 淮海工学院博士科研启动项目(KQ10130)
【CateGory Index】: R284.2
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