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《Food Science》 2012-17
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Immobilization of Lysozyme onto AB-8 Macroporous Resin and Its Enzymatic Characterization

GUO Qing-qi 1,ZHANG Na 2,FU Qun 1,TAN Dong-mei 1(1.School of Forestry,Northeast Forestry University,Harbin 150040,China;2.College of Food Engineering,Harbin University of Commerce,Harbin 150076,China)  
AB-8 macroporous resin was used as a carrier to immobilize lysozyme by glutaraldehyde cross-linking.The preparation conditions,enzymatic characteristics,microstructure and antimicrobial efficacy of immobilized lysozyme were explored.Immobilized lysozyme prepared by 4 h immobilization at 25 ℃,an enzyme-to-carrier ratio of 1:200 and a glutaraldehyde concentration of 0.3 g/100 mL presented the highest relative activity.The immobilized lysozyme showed significant improvements over free lysozyme in heat and acid tolerance.Trough a comparative analysis of their Michaelis Menten Equations,we found that immobilization resulted in a decrease in the affinity of lysozyme with chitosan,the substrate.After fifth repeated use,the immobilized lysozyme retained 57.6% of its original activity.The immobilized enzyme had good antimicrobial effect on pure milk.
【CateGory Index】: Q814.2
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