Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Food Science》 2012-17
Add to Favorite Get Latest Update

Immobilization of Lysozyme onto AB-8 Macroporous Resin and Its Enzymatic Characterization

GUO Qing-qi 1,ZHANG Na 2,FU Qun 1,TAN Dong-mei 1(1.School of Forestry,Northeast Forestry University,Harbin 150040,China;2.College of Food Engineering,Harbin University of Commerce,Harbin 150076,China)  
AB-8 macroporous resin was used as a carrier to immobilize lysozyme by glutaraldehyde cross-linking.The preparation conditions,enzymatic characteristics,microstructure and antimicrobial efficacy of immobilized lysozyme were explored.Immobilized lysozyme prepared by 4 h immobilization at 25 ℃,an enzyme-to-carrier ratio of 1:200 and a glutaraldehyde concentration of 0.3 g/100 mL presented the highest relative activity.The immobilized lysozyme showed significant improvements over free lysozyme in heat and acid tolerance.Trough a comparative analysis of their Michaelis Menten Equations,we found that immobilization resulted in a decrease in the affinity of lysozyme with chitosan,the substrate.After fifth repeated use,the immobilized lysozyme retained 57.6% of its original activity.The immobilized enzyme had good antimicrobial effect on pure milk.
【CateGory Index】: Q814.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 10 Hits
1 MA Ru HUANG Ming-zhi (College of Materials Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, China);Hydrolysis of chitosan with lysozyme[J];Journal of Beijing University of Chemical Technology;2002-06
2 XIE Xuefeng,ZHANG Zhaohui,CHEN Peice(College of Biotechnology and Environmental Engineering,Zhejiang University of Technology,Hangzhou 310014);Study on the Immobilization of Catalase by AB-8 Macroporous Absorbent Resin[J];Materials Review;2009-18
3 YU Hongfeng1 SUN Zhihao1 ZHENG Pu1 ZHANG Wei2 FAN Yunliu2 1. School of Biotechnology Southern Yangtze University, Key Laboratory of Industry Biotechnology, Ministry of Education, Wuxi 214036, China 2. Biotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, China;PREPARATION OF THE IMMOBILIZED LACTASE BY ABSORPTION WITH RESIN AND ITS OPERATIONAL STABILITY[J];Ion Exchange and Adsorption;2006-05
4 HU Ying-jun,FAN Xue-rong,WANG Qiang,WANG Ping,CUI Li(Key Laboratory of Science and Technology of Eco-Textile,Jiangnan University, Wuxi 214122,China);Immobilization of lysozyme on wool fabric[J];Wool Textile Journal;2008-10
5 Lin Qinlu et al.;Study on Elation and Purification of Lysozyme fromEgg White[J];Food Science;2002-02
6 Xie Hongwei;Improvement of Lysozyme Activity Measurement[J];Food Science;2003-09
7 YIN Jin-feng1,2,SHI Feng1,2,,WANG Xiao-yuan1,2 (1.State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China; 2.Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214122,China);Synergistic Antibacterial Effect of Lysozyme with Cell Permeabilizers on Escherichia coli[J];Food Science;2011-11
8 HU Xiao-liang,ZHOU Guo-yan(Institute of Cryomedicine and Food Refrigeration,University of Shanghai for Science and Technology,Shanghai 200093,China);Fresh-keeping Effect of Compound Sodium Alginate-Lysozyme Coating on Malu Grapes[J];Food Science;2011-20
9 LIN Xiang-yang~(1,2),HE Cheng-yun,*RUAN Rong-sheng~(1),LIU Yu-huan~(1),ZHENG Dan-dan~(1),ZHU Rong-bi~(1)(1.Institute of Biotechnology Fuzhou University,Fuzhou 350002;2.The Key Laboratory of Food Science of MOE,Nanchang University,Nanchang330047);Study on the lysozyme and its applications[J];China Food Additives;2005-06
10 MA Zheng-zhi1 HU Guo-hua1 FANG Guo-sheng2(1.Shanghai Normal University Engineering Food Research Institute,Shanghai 200234;2.Jiangsu Nantong Zhenghao Biological Technology Co.,Ltd,Nantong 226001);The advances on lysozyme research and application in China[J];China Food Additives;2007-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 HE Xiao-ning et al (Institute of Bio-engineering,Northwest A&F University,Yangling,Shaanxi 712100);Cloning and Sequencing of Human Lysozyme cDNA[J];Journal of Anhui Agricultural Sciences;2006-11
2 DAI Sheng et al(Basic Medicine and Biological Science College of Suzhou Unverisity,Suzhou,Jiangsu 215123);Effect of Sodium Fluoride on Humoral Immune Function of Silkworm Larvae[J];Journal of Anhui Agricultural Sciences;2009-15
3 WANG Da-yong et al (College of Preclinical Medicine and Biological Science,Soochow University,Suzhou,Jiangsu 215123);Effect of Fenoxycarb on Humoral Immunity of Tenebrio monitor[J];Journal of Anhui Agricultural Sciences;2010-18
4 HAN Leng, HAN Miao-jun, FENG Ting (School of Life Sciences, Wuhan University, Wuhan 430072, China);Characteristics of Lysozymes from Different Egg-White[J];Amino Acids & Biotic Resources;2004-03
5 ZHAO Jun-yan,LUO Shi-yong,XU Wen-cai(Beijing Key Laboratory of Printing & Packaging Materials and Technology,Beijing Institute of Graphic Communication,Beijing 102600,China);Research Development of Antimicrobial Packaging[J];Packaging Engineering;2012-05
6 DUAN Tao1, LUO Wei-ming2 (1. Food Science College of Southwest University, Chong Qing 400715, China; 2. Bioscience & Engineer College of Jiangxi Agriculture University, Nan Chang 330045, China);Advances in Research of Applying Biotechnology to Food Packaging[J];Packaging and Food Machinery;2008-06
7 PAN Ting-ting,CHU Yan-qiu,ZHANG Jian-xia,WU Bo,WU Liang,DING Chuan-fan (Physical-chemistry Institute,Chemistry Department,Fudan University,Shanghai 200433,China);Acetonitrile-induced Conformational Transitions of Lysozyme Studied by Electrospray Ionization Mass Spectrometry[J];Journal of Fudan University(Natural Science);2008-04
8 Wang Xiaoke, Wang Xiaoshuo, Wang Genyan, Zhang Yunling;Effect of lysozyme on growth performance of the weaned piglet[J];Feed Industry;2008-22
9 Gao Renjin Zhang yuchi(Department of Chemistry and Chemical Engineering,Minjiang University,Fuzhou,Fujian 350108);The Research Progress on carrier with Hydrogen Peroxide as Oxidant[J];Chemical Engineering & Equipment;2010-09
10 LAN Wei-qing,XIE Jing(College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China);Optimized proportion of chitosan complex biological fresh-keeping agents for the preservation of Trichiurus haumela under the cold storage[J];Journal of Fujian Agriculture and Forestry University(Natural Science Edition);2011-03
China Proceedings of conference Full-text Database 1 Hits
1 ZHAO Chao-li,XIE Wei-guo,WAN Shi-yong,WEN Feng-ping.Institute of Burn Research,the Third Hospital of Whuhan, Whuhan 430060,China;Effect of lower intensity microwave exposure on the infected wound surface in burn rat[A];[C];2011
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 GE Yu-ping et al(School of Agronomy,Ningxia University,Yinchuan,Ningxia 750002);Effect of Thickness of Preservative Film on Quality of Wolfberry (Lycium bararum L.) Fresh Fruits[J];Journal of Anhui Agricultural Sciences;2008-20
2 Wang Yujie;Lu Chibiao;Bai Guangyue;Wu Zisheng;Zhang Hezhe(Department of Chemistry,Northeast Normal University,Changchun, 130024);Catalase Immobilized with Microcapsule[J];JOURNAL OF NORTHEAST NORMAL UNIVERSITY (NATURAL SCIENCE EDITION);1994-02
3 Liu Yanru (Bioengineering College,Fujian Teachers University,Fuzhou 350007) Yu Ping (Institute of Polymer Science,Fujian Teachers University,Fuzhou 350007);The Water-soluble Chitosan Effects on Immume Function and Transplanted Tumor in Mice[J];JOURNAL OF FUJIAN TEACHERS UNIVERSITY(NATURAL SCIENCE);1999-04
4 DAI Jin-ming, ZHANG Rui-ping (College of Light & Textile Engineering & Art, Taiyuan University of Technology, Shanxi Taiyuan 030600);APPLICATION OF ANTI-BACTERIA MATERIAL IN TEXTILES[J];;2005-01
5 XUE Ping1, LU Guan-zhong2, GUO Yang-long2, WANG Yun-song2 (1. Key Laboratory of Energy Source and Chemical Engineering, Ningxia University, Yinchuan 750021,China ; 2. Research Institute of Industrial Catalysis, East China University of Science and Technology, Shanghai 200237,China));Immobilization of Polymer Supports for Penicillin Acylase[J];Journal of Functional Polymers;2005-02
6 Li Guifen and Liu Tingsong (Shandong Grape Growing and Wine-making Institute, Jinan 250100 );Advances of Research on Grape Storage-Physiology[J];JOURNAL OF FRUIT SCIENCE;2000-01
7 YE Dan, LIAN Bin Chemistry and Bio-engineering College, Gouzhou University of Technology, Guiyang Guizhou 550003, China;THE LYSOZYME AND ITS APPLICATION YE Dan, LIAN Bin[J];Guizhou Science;2003-03
8 Cao Shuxiang (Jiujiang Professional College,Jiujiang 332000);Preparation Methods for lmmobilized Enzyme[J];ENVIRONMENTAL PROTECTION OF CHEMICAL INDUSTRY;1999-05
9 DONG Xiaoyan,SHI Jinhui and SUN Yan (Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China);REFOLDING OF DENATURED-REDUCED LYSOZYME FACILITATED BY β-CYCLODEXTRIN[J];Journal of Chemical Industry and Engineering(China);2002-04
10 WANG Wei, BAI Shu, DONG Xiaoyan, SUN Yan (Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China);Effect of soluble alcohols on conformational changes and refolding of lysozyme[J];Journal of Chemical Industry and Engineering(China);2006-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved