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《Food Science》 2012-23
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Immune Modulation and Antioxidation Activity of Polysaccharides from Ganoderma atrum in Immunosuppressed Mice

WANG Jun-qiao,NIE Shao-ping,YU Qiang,YIN Peng-fei,GAO Jiang-yu,ZHANG Yi-liang,XIE Ming-yong(State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China)  
Objective: To study the immunomodulatory and antioxidant effect of polysaccharides from Ganoderma atrum in immunosuppressed mice.Methods: Immunosuppressed mouse models were established by intrapefitoneal injection of cyclophosphamid.Forty-eight mice were randomly divided into 6 groups: blank control,positive control,model control,and high-,medium-and low-dose Ganoderma atrum polysaccharide groups.Ganoderma atrum polysaccharides were administrated by gavage at doses of 100,50 mg/(kg.d) and 25 mg/(kg.d) to mice for 7 consecutive days,respectively.At 24 h after the last administration,the mice in each group was sacrificed and spleens were collected for the measurement of spleen index and the levels of spleen super-oxide dismutase(SOD),catalase(CAT),maleic dialdehyd(MDA) and total antioxidant capacity(T-AOC).Moreover,T and B lymphocyte proliferation was analyzed by MTT and the levels of TNF-α and IL-2 in spleen homogenate supernatant were assayed by ELISA.Results: Compared to control mice,Ganoderma atrum polysaccharides significantly improved mouse spleen index,promoted T and B lymphocyte proliferation,increased SOD,CAT and T-AOC levels,and reduced MDA content in mouse spleen,while only high and medium-dose groups indicated obviously improved TNF-α and IL-2 levels in spleen cells.Conclusions: Ganoderma atrum polysaccharides can effectively antagonize cyclophosphamideinduced immune suppression in mice and enhance antioxidant function in immunosuppressed mice.
【Fund】: 国家自然科学基金项目(31071532;31130041);; “十二五”国家科技支撑计划项目(2012BAD33B06);; 食品科学与技术国家重点实验室目标导向项目(SKLF-MB-201001)
【CateGory Index】: R285.5
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