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《Food Science》 2013-01
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Purification and Identification of Nucleoside Components in Cordyceps cicadae Fruit Bodies

CHEN An-hui,CHEN Hong-wei,XU Yang,ZHUO Tao,SHAO Ying(Department of Food and Biology,Xuzhou Institute of Technology,Xuzhou 221008,China)  
The aim of this study was to extract,purify and identify nucleoside components of Cordyceps cicadae fruit bodies.Samples were extracted with 65% ethanol by ultrasonic-assisted extraction,and a supernatant was obtained from the resulting extract by centrifugation at 10000 r/min for 5 min and separated by RP-HPLC using 0—100% methanol as a mobile phase through gradient elution at a flow rate of 15 mL/min(1 mL injection volume,260 nm detection wavelength).As a result,6 final products were obtained and identified as cytosine,uridine,inosine,guanosine,adenosine and cordycepin,respectively,with a purify exceeding 95%.This study demonstrates that RP-HPLC is a feasible,simple and accurate technique for separating and preparing nucleoside components of Cordyceps cicadae fruit bodies.
【Fund】: 江苏省科技计划项目(BN2011017;BN2011022);; 国家星火计划项目(2010GA730017)
【CateGory Index】: S567.35
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