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《Food Science》 2014-08
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Determination of Pantothenic Acid in Formula Milk Powder Using Ultra Performance Liquid Chromatography-Isotope Dilution Mass Spectrometry

QU Yan;CUI Ya-juan;LI Quan-xia;CHEN Zhao-tian;LI Dong;LIANG Min-hui;Beijing Research Institute for Nutritional Resources;College of Life Science and Bio-enginee ring, Beijing University of Technology;  
An ultra performance liquid chromatography-isotope dilution mass spectrometry(UPLC-IDMS) method has been developed for the determination for pantothenic acid(PA) in formula milk powder. Samples were extracted with ammonium acetate solution, and precipitated protein by adding chloroform for analyzing by UPLC-MS-MS. The analyte was separated using an HSS T3 column. The mobile phase consisted of 10 mmol/L ammonium acetate with 0.1% formic acid and acetonitrile. The PA was identified by multiple reaction monitoring(MRM) and quantified by internal standard method. The results showed that the limit of quantification for PA was 0.040 mg/100 g. The recoveries at three spiked levels and the relative standard deviations were 96.9%–104.3% and 3.78%–5.04%, respectively. This method proved to be simple, timesaving, sensitive and accurate for the determination of PA in formula milk powder.
【Fund】: 北京市科技新星计划项目(2011059);; 卫生行业科研专项项目(201202012);; 北京市科委“双十计划”项目(Z121106002812109);; 北京市优秀人才培养资助项目(2011D002022000001)
【CateGory Index】: O657.63;TS252.7
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