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《Food Research and Development》 2006-10
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THE STUDY ON DETERMINATION OF β-GLUCAN BY FLUORIMETRY WITH SODIUM FLUORESCEIN

LIAN Xi-jun, LU Xiao-xiang, LIU Qin-sheng, ZHAO Guang-li, XI Jun (College of Biotechnology and Food Science, Tianjin University of Commerce,Tianjin 300134,China)  
Determination of β-glucan in carbohydrates was studied by detecting change of exciting wavelength and emission wavelength as sodium fluorescein was mixed with β-glucan, glucose, fructose and maltose. The results showed that if sodium fluorescein was mixed with β-glucan, UV-absorption of fluorescein sodium changed from 436.5 nm to 510.6 nm, fluorescence absorption also changed: exciting wavelength from 487.3 nm to 475.9 nm, emission wavelength from 517.6 nm to 510.6 nm, if sodium fluorescein was mixed with other carbohydrates such as glucose, fructose or maltose, no change happened for fluorescence absorption. The optimal proportion of sodium fluorescein and β-glucan was 6:1, the linear concentration range was 0.1 μg/mL~0.25 μg/mL, the regression equation: y = -2.014x + 1.075 7,R2 = 0.997 8, the purity of β-glucan in raw material was 95 % using such equation, comparing with 96 % by standard enzyme method.
【CateGory Index】: TS201.2
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