COMPOSITION AND STRUCTURE ANALYSIS OF GLYCOPROTEIN FROM JELLYFISH ORAL-ARMS
REN Guo-yan1, LI Ba-fang2, HOU Yu-ze1, ZHAO Sheng-juan1, LIANG Hua1, CHEN Xiu-jin1 (1.Food and Bioengineering Department , Henan University of Science and Technology , Luoyang 471003, Henan, China; 2. Faculty of Science and Technology, Ocean University of China ,Qingdao 266003, Shandong, China)
A homogeneous Glycoprotein (JGP - Ⅲ ) was isolated and purified from jellyfish oral -arms. The molecular weight of JGP-Ⅲ was estimated to be 109.7 ku by SDS-PAGE. JGP-Ⅲ contain 12.61 % total suger, 74.34 % protein, 8.47 % amide sugar,0.84 % uronic acid, 1.06 % sulfate group and 0.92 % sialic acid. Monosaccharides composition of JGP-Ⅲwas Rha, Fuc, Ara, Man, Glc, GalNAc, and GlcNAc determined by GC. Amino acid composition of JGP-Ⅲ was rich in glycine, valine, glutanmic acid, alanine, proline, methionine and asparagic acid, and lacking in histidine and tryptophan. The existence of O-glycosidic and N-glycosidic linkage in the glycoprotein was demonstrated with β–elimination reaction and peptide N-glycosidase F reaction. IR spectrum of JGP-Ⅲindicated the strcture characterization of glycoprotein.
【CateGory Index】： TS254.1