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《Food Research and Development》 2013-16
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Optimization of Extraction Process by Using Ultrasound-assisted Methodology and Antioxidant Activity of Polysaccharide from Stropharia rugosoannulata

DU Min-hua;WANG Xiao-li;SU Hai-fei;LIU Ming-xing;College of Life Science and Technology ,Nanyang Normal University;  
In this study, the process conditions for extracting polysaccharides from Stropharia rugosoannulata with the assistance of ultrasound were optimized by L9(34) orthogonal array design methods. The optimal extraction process conditions were determined as follows: extraction temperature, 65 ℃; ultrasound power, 600W; and material/liquid ratio, 1 ∶ 35(g/mL) for an extraction duration of 1.0 h. Under these conditions, the extraction yield of polysaccharides from Stropharia rugosoannulata was 8.16 %. The decreasing order of importance of the four process conditions affecting polysaccharide extraction was extraction duration, extraction temperature,ultrasound power and material/liquid ratio. The scavenging ability of hydroxyl radicals and superoxide anion radicals of Stropharia rugosoannulata polysaccharide were assayed by chemiluminescence.There sults showed that Stropharia rugosoannulata polysaccharide have evident abilities of scavenging hydroxyl radicals and superoxide anion radicals.The crude Stropharia rugosoannulata polysaccharides obtained had strong superoxide anion radicals scavenging effect, with an IC50of 108.60 μg/mL, which was more than 3.2-fold increased after hydroxyl radicals, revealing an IC50of 345.98 μg/mL.
【Fund】: 河南省教育厅自然科学研究计划项目(2007550012);; 南阳师范学院STP项目(2010002)
【CateGory Index】: S646
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