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Genetic Diversity Analysis of Pueraria in Guangxi Based on SCoT Markers

SHANG Xiaohong;YAN Huabing;CAO Sheng;XIAO Liang;WANG Yan;OU Kunpeng;Cash Crops Research Institute,Guangxi Academy of Agricultural Sciences;Guangxi Crop Genetic Improvement and Biotechnology Key Lab;  
In order to analyze the genetic similarity of 44 Pueraria germplasm resources in Guangxi,the DNA of the germplasm resources was amplified by SCoT primers.The results showed that,25 SCoT primers produced 233 bands,among which 194 bands were polymorphic,the percentage of polymorphic rate was up to 86.99%,and the average polymorphic number was 7.76 per primer.The cluster analysis showed that the range of genetic similarity(GS)was 0.587 to 0.982 among 44 Pueraria samples,the samples could be divided into 2 groups at the GS of 0.65,the 37th germplasm alone was clustered into one group,and the rest accessions was clustered into another group.All of the accessions could be clustered into six groups at the GS of 0.74.The result revealed that SCoT molecular markers are suitable for the analysis of genetic diversity of the Pueraria germplasm resources,and will lay a foundation for the evaluation and selection of the germplasm resources of Pueraria in Guangxi.
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