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Study on the Characteristics and Applications of a New Hydrophobic Interaction Chromatographic Packing Using Chitosan as Support

WANG Yun,\ GUO Min liang,\ JIANG Shou lei,\ CHEN Tian, JIANG Yong ming, CHEN Yun (College of Bioscience & Biotechnology,Yangzhou University,Yangzhou 225009, China)  
Based on chitosan,by using valeraldehyd as ligand,a new hydrophobic interaction chromatographic (HIC) packing material was prepared by an improved method.The effects of salt concentration, temperature,pH on the adsorption of bovine serum albumin(BSA) were investigated.The results showed that the adsorption behavior of protein by packing material is in agree with the theory of hydrophobic interaction.The α amylase could be separated by HIC with high recovery of enzymatic activity.
【Fund】: 江苏省科委“九五”农业重点攻关项目! (BE96443)
【CateGory Index】: O657.7
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Chinese Journal Full-text Database 5 Hits
1 WANG Yun 1 , WANG Zheng 2 (1.Department of Biochemistry,Changshu College,Changshu 215500; 2.College of Bioscience & Biotechnology,Yangzhou University 225009);Applications of Hydrophobic Interaction Chromatography in Biochemical Research[J];JOURNAL OF CHANGSHU COLLEGE;2000-04
2 WANG Zheng, GUO Min liang, JIANG Yong ming, WANG Yun, JIANG Shou lei, CHENG Yun (College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009,China);Study on the Synthesis of a Novel Packing Material —Butyl Cross-linked Chitosan for Hydrophobic Interaction Chromatography[J];Journal of Functional Polymers;2001-01
3 Xia Caifen Xing Dongyang Chen Bingren(Department of Chemistry,South China Normal University,Guangdong Guangz hou 510631,China);Preparation and Development of Crosslinked Chitosan[J];Chemical Industry Times;2004-02
4 FENG Changgen1 BAI Linshan1 REN Qisheng2 1 Center of Research and Development for New Pharmaceuticals, Beijing Institute of Technology, Beijing 100081, China 2 Beijing Jiangzhong Institute of Pharmaceuticals, Beijing 100050, China;CHITOSAN AND ITS DERIVATIVE ADSORBENTS IN THE APPLICATION OF SEPARATION AND PURIFICATION OF BIOPOLYMERS[J];Ion Exchange and Adsorption;2003-03
5 WANG Yun 1,CHANG Ling 2,WU Jin nan 1 (1.Department of Biology and Chemistry;2.Personnel Division;Changshu College,Changshu 215500,China);Hydrophobic interaction chromatography and its application in purification of biopolymers[J];Yantai Teachers College Journal(Natural Science);2002-02
Chinese Journal Full-text Database 1 Hits
1 Bai Quan, Wei Yinmao and Geng Xindu * (Institute of Modern Separation Science, Northwest University, Xi′an, 710069);Investigation of α Amylase Denatured by Urea and Guanidine Hydrochloride I. Studies on Denaturation Mechanism and Renaturation Efficiency by High Performance Hydrophobic Interaction Chromatography[J];CHINESE JOURNAL OF CHROMATOGRAPHY;1997-04
Chinese Journal Full-text Database 10 Hits
1 CHEN Hua-you et al(Institute for Biological Sciences,Jiangsu University,Zhenjiang,Jiangsu 212013);Strategies on the Separation and Purification of Protein[J];Journal of Anhui Agricultural Sciences;2009-36
2 YU Xiao Ling, MI Li, CHEN Zhi Nan 863 Hepatoma Drug Targeting Project Group, Department of Pathology, Faculty of Preclinical Medicine, Fourth Military Medical University, Xi'an 710033, China;Single step preparation and purification of F(ab') 2 of anti-hepatoma mAb HAb18 for clinical application[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;2000-01
3 ZHU Li-Guo,REN Fu-Ping,CHEN Wu-LingCollege of Life Science,Northwest University,Xi'an 710069,China;Fmoc solid-phase synthesis of MSH peptide[J];Journal of the Fourth Military Medical University;2006-22
4 LI Lin,LI Jinnian,YU Weiyi(Anhui Agriculture University, Hefei ,Anhui, 230036,China);Progress in Determination Methods of the G+C Content of Bacteria[J];Progress In Veterinary Medicine;2003-01
5 YU Yan-ling~(1,2), WANG Yao-hua~(1), LIAN Yi-ping~(1), HUANG Zhen-yu~(1), YANG Peng-yuan~(1) (1. Department of Chemistry, Fudan University, Shanghai 200433, China; 2. College of Pharmacy, Second Military Medical University, Shanghai 200433, China);Investigations of Proteome Affinity Isolation(Ⅰ) Study on Optimal Condition for Protein Biotinylation at Free Amino Groups[J];Journal of Fudan University;2003-06
6 SHI Zhixian\+1 HU Fengzu\+1 SHAO Yun\+1 FAN Limin\+2 \+1 (Northwest Plateau Institute of Biology, the Chinese Academy of Sciences Xining 810001) \+2 (Lanzhou Institute of Chemical physics, the Chinese Academy of Sciences Lanzhou 730000);Characteristics of Selective and Nonadsorptive of Proteins on Ion Exchange Chromatography[J];ANALYSIS AND TESTING TECHNOLOGY AND INSTRUMENTS;1999-04
7 SHI Zhi xian\+1, FAN Li min\+2 (1. Northwest Plateau Institute of Biology, the Chinese Academy of Sciences, Xining 810001, China; 2. Lanzhou Institute of Chemical Physics, the Chinese Academy of Sciences, Lanzhou 73000, China);Preparative High Performance Liquid Chromatography for Proteins[J];Analysis and Testing Technology and Instruments;2000-04
8 SONG Ya li, HU Feng zu, LIU mei, SHI Zhi xian (Northwest Plateau Institute of Biology, CAS Xining 810001; China);Analysis of Nucleosides in DNA and RNA of E.coli by RP-HPLC[J];Analysis and Testing Technology and Instruments;2002-02
9 Wei Yun; Fan Limin; Shen Runnan; Zhao Liang (Lanzhou, Institute of Chemical Physics, Chinese Academy of Sciences. Lanzhou 730000 );Preparation of a Novel Macroporous Silicagel-Polymer-Bonded Packing and Its Application to the Separation of Protein[J];Chinese Journal of Analytieal Chemistry;1996-10
10 Wei Yun, Fan Limin, Chen Liren (Lanzhou Insitute of Chemical Physiscs, Chinese Academy of Sciences, Lanzhou 730000);Effect of Different Pore Diameter Silica-based Polymer-bonded Phases on Separation of Proteins[J];Chinese Journal of Analytieal Chemistry;1997-09
Chinese Journal Full-text Database 10 Hits
1 WANG Yun et al (College of Biological Science & Technology,Yangzhou University,Yangzhou,Jiangsu 225009);Preliminary Study on Chitosan as a Hydrophobic Interaction Chromatographic Matrix[J];Journal of Anhui Agricultural Sciences;2005-07
2 CHEN Chun yun 1, ZHUANG Yuan yi 1,LIU Fei 1,YANG Min 2 (1 Department of Environmental Science, Nankai University, Tianjin 300071,China; 2 Research Center for Eco Environmental Sciences, Chinese Academy of Sciences, Beijing 100085,China);ADSORPTION EQUILIBRIUM AND KINETIC OF DYES ONTO DRIED ACTIVATED SLUDGE[J];Journal of Safety and Environment;2003-03
3 LIN Jian,GE Sheng-xiang,WANG Ying-bin,LUO Wen-xin,WU Ting, LI Shao-wei,CHENG Tong,ZHANG Jun.XIA Ning-shao ( The Research Center for Molecular Virology of Fujian Province , The Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering , Xiamen University, Xiamen 361005 , China );Prokaryotic Expression of Different Regions of SARS-CoV Nucleocapsid Protein[J];Chinese Journal of Virology;2005-02
4 WU Jing\|ping(Normal College of Beijing Union University, Beijing\ 100011,China);Improved Method for Acid-base Titration Determination of the Degree of N-acetylation of Chitosan[J];Journal of Beijing Union University;2003-03
5 Xu Changfa;Wang Lanxian;Liu Defu;Ma Xiaolan(Department of Biology,Beijing University,100871)(College of Sciences and Humanities of BUU,100083)[;The Isolation,Purification and Characterization of Thrombin from Porcine Plasma[J];JOURNAL OF BEIJING UNION UNIVERSITY;1994-01
6 HE Yan,QI Hong(Changchun Education Institute, Jilin, Changchun 130061,China);Reversible Catalytic Reactions[J];Journal of Changchun Teachers College;2005-07
7 Zhang Yankun;Liu Guozhong(Institute of Hygiene and Evironmental Medicine,Academy ofMilitary Medical Sciences, Tianjin, 300050 );The Preparation and Applications of Chitin,Chitosan and their Derivatives[J];CHINA SURFACTANT DETERGENT & COSMETICS;1998-04
8 LOU Dao-cheng, YI Ping-gui, LIU Jun-feng, HU Zhong-yu (Department of Chemical Engineering, Xiangtan Polytechnic University, Xiangtan 411201, China);Study on Adsorbing of Heavy Metal Ions in Electroplating Waste Water by Modified Chitosan[J];Materiais Protection;2002-01
9 Z E N G Lian┐ying( Zhejiang University, Hangzhou 310027, China);The Developm entof Study on Chitooligosaccharides[J];MATERIALS SCIENCE AND ENGINEERING;1999-03
10 JU Hong_fang(1.Changshu Institute of Technology,Changshu 215500,China;2.Suzhou University,Suzhou 215000,China);Preparation of Chitosan modified with Schiff Base Type Macrocycles[J];Journal of Changshu College;2005-02
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 ZHU Hua-yue et al (Department of Environmental Engineering,Taizhou University,Linhai,Zhejiang 317000);Study on the Adsorptiona and Removal of Protonated Cross-linked Chitosan Granules to Nitrate in Water Body[J];Journal of Anhui Agricultural Sciences;2008-07
2 TIAN Miao-Miao,SU Ri-Yan,JIA Qiong*,BAO Chang-Li,QUAN Xin-Jun(College of Chemistry,Jilin University,Changchun 130022);Spectrophotometric Determination of Lysozyme by On-line Preconcentration with A Microcolumn Containing La~(3+)-TiO_2-Zeolite[J];Chinese Journal of Analytical Chemistry;2011-01
3 CHEN Fei, LUO Guangsheng, WANG Yujun (State Key Lab of Chemical Engineering, Department of Chemical Engineering Tsinghua University, Beijing 100084);STUDIES ON ADSORPTION PROPERTIES OF CHEMICALLY MODIFIEDCHITOSAN RESINS TO DIURETICS[J];Acta Polymerica Sinica;2005-01
4 WANG Yu ting, LIU Yu hong, ZHANG Shu qin (Department of Environmental Science, School of Resource and Environmental Science, Wuhan University, Wuhan 430072,China);Advances in Chemical Modofication and Application of Chitin, Chitosan and their Derivatives[J];Journal of Functional Polymers;2002-01
5 Zhang Ying Su Liqiang (College of Chemistry and Chemical Engineering,QiqiharUiniversity,Heilongjiang Qiqihar 161006);Selective Enrichment of Iysozyme from the Protein Samples in the Molecularly Imprinted Polymer System[J];Chemical Industry Times;2010-08
6 YING Guoqing,LU Xia,YI Yu(College of Pharmaceutical Science,Zhejiang University of Technology,Hangzhou 310014,Zhejiang,China);Preparation of novel aminated chitosan adsorbent and its adsorption characteristics[J];Chemical Industry and Engineering Progress;2007-02
7 YU Shao-ying (Fuzhou Technology College,Fuzhou 350000,China);Study on the adsorption of metal ion by cross-linked chitosan[J];Journal of Jilin Institute of Chemical Technology;2007-04
8 FENG Changgen1 BAI Linshan1 REN Qisheng2 1 Center of Research and Development for New Pharmaceuticals, Beijing Institute of Technology, Beijing 100081, China 2 Beijing Jiangzhong Institute of Pharmaceuticals, Beijing 100050, China;CHITOSAN AND ITS DERIVATIVE ADSORBENTS IN THE APPLICATION OF SEPARATION AND PURIFICATION OF BIOPOLYMERS[J];Ion Exchange and Adsorption;2003-03
9 WEI Xian-hang1,2,QU Li-jun1,2,GUO Xiao-qing1,2(1.Qingdao University,Qingdao 266071,China,2.Laboratory of New Fiber Materials and Modern Textile,the Growing Base for State Key Laboratory,Qingdao 266071,China);Characterization and Absorption Studies of the Reactive Dye on Cross-linked Chitosan Resins[J];Shandong Textile Science & Technology;2010-04
10 YING Guo-qing,LU Xia,SHI Lu-e,YI Yu,MEI Jian-feng(School of Pharmaceutical Science,Zhejiang University of Technology,Hangzhou 310032,China);Preparation of novel chitosan microsphere and the adsorption proterties for flavones[J];Chinese Journal of Biochemical Pharmaceutics;2007-02
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 Chang Jianhua GengXindu and Yin Jianning,Laboratory of Modern Separation Science,Department of Chemistry,Northwest University,Xi'an 710069;Study on Synthesis and Characteristics of Packings of High Performance Hydrophobk Interaction Chromatography[J];Chinese Journal of Chromatography;1991-04
2 Cheng Keqin;Dang Xinrang and Li Huaru(Department of Chemical Engineering,Northwest University,Xi'an,710069);Studies on Denaturation Kinetics of a-Amylase by Chromatography[J];CHINESE JOURNAL OF CHROMATOGRAPHY;1995-01
3 Li Huaru, Dang Hongxi (Laboratory of Modern Separation Science);The Determination of Biological Activity and Recovery for α-amyiase Purified by HPAC[J];Journal of Northwest University(Natural Science Edition);1991-02
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