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《CHINESE JOURNAL OF CHROMATOGRAPHY》 2000-04
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Study on the Characteristics and Applications of a New Hydrophobic Interaction Chromatographic Packing Using Chitosan as Support

WANG Yun,\ GUO Min liang,\ JIANG Shou lei,\ CHEN Tian, JIANG Yong ming, CHEN Yun (College of Bioscience & Biotechnology,Yangzhou University,Yangzhou 225009, China)  
Based on chitosan,by using valeraldehyd as ligand,a new hydrophobic interaction chromatographic (HIC) packing material was prepared by an improved method.The effects of salt concentration, temperature,pH on the adsorption of bovine serum albumin(BSA) were investigated.The results showed that the adsorption behavior of protein by packing material is in agree with the theory of hydrophobic interaction.The α amylase could be separated by HIC with high recovery of enzymatic activity.
【Fund】: 江苏省科委“九五”农业重点攻关项目! (BE96443)
【CateGory Index】: O657.7
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