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《Chinese Journal of Chromatography》 2001-04
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Simultaneous Purification and Renaturation of Recombinant Human Interferon-Alpha Expreessed by E.coli by High-Performance Hydrophobic Interaction Chromatography

GUO Li an (Chemistry Department, The Fourth Military Medical University of PLA, Xi'an 710032, China)  
One step simultaneous purification and renaturation of interferon alpha from the inclusion body of E coli expreessed by high performance hydrophobic interaction chromatography(HPHIC) are presented The inclusion body was dissolved by 8 mol/L guanidine hydrochloride The sample lysis was centrifuged and the solution was renaturated by three different methods, i e hydrophobic interaction chromatography, dilution and dialysis The total activity yield renaturation by hydrophobic interaction chromatography was 2 times and 2 6 times as much as that by dilution and by dialysis The purity of the target product was 95% according to high performance size exclusion chromatography and the specific activity was 1 8×10 8 IU/mg The method developed is simpler and more efficient than others
【Fund】: 国家自然科学基金资助项目 (批准号 :30 0 80 0 0 7);; 高等学校骨干教师计划资助项目 (批准号 :2 0 0 0 65 66)
【CateGory Index】: O658
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【References】
Chinese Journal Full-text Database 1 Hits
1 LI Ying-Tao(Shanghai Hualian Pharmaceutical Co. Ltd., Shanghai 200083);Purification of Recombinant Human Interferon α-2b by Two-step Column Chromatographic Process[J];Chinese Journal of Pharmaceuticals;2004-03
【Citations】
Chinese Journal Full-text Database 4 Hits
1 GUO Li-An;ZHU Bao-Quan(The Centre Laboratory, Fourth Military MedicalUniversity, Xi'an 710033, China,) ZCHEN Dai--Jie( Shanghai Institute of Pharmaceutical Industry, China);Purification of recombinant human interferon-α by magnetic affinity microsphere[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;1999-01
2 GUO Li\|An\ GENG Xin\|Du (Institute of Mondern Separation Science,Northwest University,Xi'an\ 710069);Simultaneous Renaturation and Purification of Proteins by Liquid Chromatography[J];CHINESE JOURNAL OF BIOTECHNOLOGY;2000-06
3 LIU Tong , GENG Xin du (Institute of Modern Separation Science, Shaanxi Provincial Key Laboratory of Modern Separation Science, Northwest University, Xi'an 710069, China);The Application of Liquid Chromatography in Genetic Engineering[J];Chinese Journal of Chromatography;2000-01
4 GUO Li An, ZHU Bao Quan a, CHEN Dai Jie a (The Centre Labaratory, 4th Military Medical University, Xi'an 710032; aShanghai Institute of Pharmaceutical Industry, Shanghai 200040);SELECTION OF REFOLDING CONDITIONS OF RECOMBINANT PROTEINS BY USING THE THREE STATE MODEL[J];CHINESE JOURNAL OF PHARMACEUTICALS;1999-03
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 XIA Qi-yu et al(Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101);Research Progress in Renaturation of Inclusion Body Protein[J];Journal of Anhui Agricultural Sciences;2008-14
2 GUO Li-An;ZHU Bao-Quan(The Centre Laboratory, Fourth Military MedicalUniversity, Xi'an 710033, China,) ZCHEN Dai--Jie( Shanghai Institute of Pharmaceutical Industry, China);Purification of recombinant human interferon-α by magnetic affinity microsphere[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;1999-01
3 GUO Li An 1, DONG Jian Zhong 2 1Central Laboratory, Faculty of Preclinical Medicine,Fourth Military Medical University, Xi'an 710033, China, 2Henan Normal University, Xinxiang 453002;Preparation of HPAC and purification of rhIL-2[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;2000-10
4 HUANG Ya-Yu 1,TONG Wei 2,GUO Li-An 3 1Department of Hematology,Xijing Hospital, 2Dean’s Office, 3Department of Chemistry,School of Basic Medicine,Fourth Military Medical University,Xi’an 710033,China;Refolding of ten denatured standard proteins by high performance hydrophobic interaction chromatography[J];Journal of The Fourth Military Medical University;2005-08
5 GENG Xin du 1 ,WANG Chao zhan 1,ZHANG Yang jun 1,SHEN Ye hua 1,WANG Da wei 2,TIAN Bo 2 (1.Institute of Mordern Separation Scienec,Northwest University,Xi’an 710069,China; 2.Laboratory of Molecular Virus and Bioengineering,Institue of Micro;The purification of recombinant bovine mature PrP~c by high performance hydrophobic lnteraction chromatography[J];Basic Sciences Journal of Textile Universities;2001-01
6 Liang Feng, Guo Zhian, Gao Junping, Chang Jianhua (Department of Chemistry, Northwest University, Xi'an 710069);Separation of Protein With Multiple Injection on High-Performance Ion -Exchange Chromatography[J];Chinese Journal of Analytieal Chemistry;1998-08
7 Gan Hongyu, Shang Zhenhua, Liu Xueliang, Wang Junde (Dalian Institute of Chemical Physic, Chinese Academy of Sciences, Dalian 116012);Study of Isothermal Adsorption of Bovine γ-Globulin on Nylon Affinity Membrane with L-Tryptophane Ligand by Batch Method[J];Chinese Journal of Analytieal Chemistry;2001-06
8 Shen Yehua, Wang Haibo, Wei Yinmo, Geng Xindu * (Institute of Modern Separation Science, Key Laboratory of Modern Separation Science in Shaanxi Province, Northwest University, Xi′an 710069);Studies on the Assisted Actions of Furfuryl Alcohol for the Renaturation Efficiency of α-Chymotrysin by Liquid Chromatography[J];Chinese Journal of Analytieal Chemistry;2002-12
9 BAI Quan *, KONG Yu, GENG Xin Du(Institute of Modern Separation Science, Key Lab of Separation Science in Shaanxi Province, Northwest University, Xiaan 710069, China);Studies on the Refolding of Reduced-Denaturated Insulin with High Performance Hydrophobic Interaction Chromatography[J];Chemical Research In Chinese Universities;2002-08
10 ZHANG Yanmin;LI Cheng;WU Yao;LIANG Wenjing;SONG Wanlin;YAO Peng;CONG Lina;LIU Zhiwen;School of Biological Engineering,Dalian Polytechnic University;;Refolding of recombinant Fenneropenaeus chinensis lysozyme inclusion body[J];Journal of Dalian Polytechnic University;2014-04
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LI Feng-ling et al (Rice Research Institute of Sichuan Agricultural University,Wenjiang,Sichuan 611130);Plant Lectin and its Application in the Genetic Engineering of Plant Resistance to Insects[J];Journal of Anhui Agricultural Sciences;2006-03
2 GAO Jin-rong,WU Zheng-hui,SHE Ying-long,YE Li,YE Lin-baiState key Laboratory of Virology,College of Life Sciences,Wuhan University,Wuhan 430072,China;Effects of Arginine,Arginine Hydrochloride,Cysteine,Cystine on tPA Renaturation[J];Amino Acids & Biotic Resources;2006-03
3 Zhang Dezhen Hou Yunde Wu Shuhua Su Chengzhi (Institute of Virology, Chinese Academy of Preventive Medicine,Beijing);PURIFICATION OF RECOMBINANT HUMAN INTERFERON GAMMA[J];Chinese Journal of Virology;1989-01
4 HOU Yi long 1,ZHANG Kai chun 2,HU Wen yu 3,WU Lu ping 3,LIN Ke 2,YIN Shu ping 2 (1.Department of Bioengineering,Dalian University,Liaoning, Dalian 116622,China; 2.Beijing Institute of Forestry and Pomology,Beijing 100093,Chin;Detection of Several RNA Viruses in Fruit Trees by RT-PCR[J];Chinese Journal of Virology;2002-01
5 Liu Hongyan,Ma Yanbing,Li Zhihua,Ji Qiuyan,Li Jianfeng,Zhou Li,Guo Ren,Dai Changbai (Institute of Medical Biology,CAMS and PUMC,Kunming);Cloning expression and purification of human interleukin 6[J];JOURNAL OF NORMAN BETHUNE UNIVERSITY OF MEDICAL SCIENCE;1999-01
6 LAN Jian, SHAO Sheng rong, YAO Ai xing (Institute of Grassland Science, Ningxia Agricultural College, Yinchuan 750105, China);Studies on seed productivity of turf type perennial ryegrass[J];PRATACULTURAL SCIENCE;1999-05
7 GUO Li-An;ZHU Bao-Quan(The Centre Laboratory, Fourth Military MedicalUniversity, Xi'an 710033, China,) ZCHEN Dai--Jie( Shanghai Institute of Pharmaceutical Industry, China);Purification of recombinant human interferon-α by magnetic affinity microsphere[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;1999-01
8 GUO Li An 1, DONG Jian Zhong 2 1Central Laboratory, Faculty of Preclinical Medicine,Fourth Military Medical University, Xi'an 710033, China, 2Henan Normal University, Xinxiang 453002;Preparation of HPAC and purification of rhIL-2[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;2000-10
9 HUANG Ya-Yu 1,TONG Wei 2,GUO Li-An 3 1Department of Hematology,Xijing Hospital, 2Dean’s Office, 3Department of Chemistry,School of Basic Medicine,Fourth Military Medical University,Xi’an 710033,China;Refolding of ten denatured standard proteins by high performance hydrophobic interaction chromatography[J];Journal of The Fourth Military Medical University;2005-08
10 WANG Li-Li, GENG Xin-Du Institute of Modern Separation Science, Shaanxi Provincial Key Lab of Modern Separation Science, Northwest University, Xi'an 710069, China;Urea gradient SEC in renaturation with simultaneous purification of rhSCF[J];Journal of The Fourth Military Medical University;2005-15
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 WANG LiLi & GENG XinDu Key Laboratory of Synthetic and Natural Functional Molecular Chemistry of Ministry of Eduction, Institutr of Modern Separation Science, Northwest University, Shaanxi Key laboratory of Modern Separation Science, Xi’an 710068, China;Recent development of the expression, renaturation and purification of proteins sourcing from E.coli[J];Science in China(Series B:Chemistry);2009-08
2 Luo Zunxi,Yang Zhicai,Lu Sushan,Wu Zhuandi,Feng Cuiliang,Yu Shizhou,Yang Bengpeng,Zhang Shuzhen Institute of Tropical Biosciences and Biotechnology/ Ministry of Agriculture Key Biotechnology Laboratory for Tropical Crops,CATAS,Haikou 571737,China;Genetic Transformation of Pokeweed Antiviral Protein Gene into Sugarcane[J];Chinese Journal of Tropical Crops;2009-11
3 LI Yan-li;TAO Jie;ZHANG Xin-jun;WANG Yin;ZHU Guo-qiang;Collage of Veterinary Medicine,Yangzhou University;;PROKARYOTIC EXPRESSION OF BOVINE HERPESVIRUS-1 GB GENE[J];Chinese Journal of Animal Infectious Diseases;2013-05
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 LIU Yuan-Kun, GUO Li-An, LIANG Guo-Dong and ZHUANG Wen-Xu Central Laboratory, Tangdu Hospital Department of Biology;Preparing magnetite albumin microspheres containing adriamycin[J];Journal of The Fourth Military Medical University;1991-05
2 Shi Yali,Ma Feng, Geng Xindu (Institute of Modern Separation Science, Northwest University, Xi' an 710069);A New Characterization Parameter Z in Liquid Chromatogaphy Ⅳ. The Relationship Between Concentration of Formic Acid and Molecular Conformation of Biopolymers in Reversed-phase Liquid Chromatography[J];Chinese Journal of Analytieal Chemistry;1994-07
3 GUO Li-An; LIU Shu-Lin(The Centre Labaratory, 4th Mlitary Medical University, Xi'an, 710032)ZHU Bao-Quan(Shanghai Institute of Pharmaceutical Industry, Shanghai, 200040);Purification of Recombinant Human Interferon-γ by High-Performance Hydrophobic Interaction Chromatography and Size Exclusion Chromatography[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1997-06
4 BAI Quan, WEI Yin-Mao, GENG Ming-Hui, GENG Xin-Du (Institute of Modern Separation Science, Northwest University, Xi'an, 710069);Studies on the Various Refolded Intermediates of α-Amylase Denatured by Urea with High Performance Hydrophobic Interaction Chromatography[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1997-08
5 GUO Li-An(The Centre Laboratory, 4th Military Medical University, Xi'an, 710032)ZHU Bao-Quan(Shanghai institute of Pharmaceutical industry, Shanghai, 200040);Synthesis of High Efficiency Packing and Using for Recombinant Human Interferons Separation in High-performance Hydrophobic Interaction Chromatography[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1997-09
6 SHI Ya-Li;MA Feng;GENG Xin-Du(Institute of Modern Separation Science,Northwest University,Xi'an,710069);A New Characterization Parameter for Molecular Conformation Change of Proteins in Reversed-phase Liquid Chromatography[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1994-09
7 LIU Tong , GENG Xin du (Institute of Modern Separation Science, Shaanxi Provincial Key Laboratory of Modern Separation Science, Northwest University, Xi'an 710069, China);The Application of Liquid Chromatography in Genetic Engineering[J];Chinese Journal of Chromatography;2000-01
8 Yang Qing(Military Medical Institute of Shenyang Military Region, Shenyang, 110031);Purification of Recombinant Human Interleukin-2 by Large-Scale Preparative Reversed-Phase High Performance Liquid Chromatography[J];CHINESE JOURNAL OF CHROMATOGRAPHY;1996-05
9 Xiong Bohui and Wang Junde (Dalian Institute of Chemical Physics, the Chinese Academy of Sciences, Dalian, 116012);Developments and Applications of Perfusion Chromatography[J];CHINESE JOURNAL OF CHROMATOGRAPHY;1997-06
10 Liu Tong and Geng Xindu * (Institute of Modern Separation Science, Northwest University, Xian, 710069);New Development of Hydrophobic Interaction Chromatography and Its Applications in Biochemical Research[J];CHINESE JOURNAL OF CHROMATOGRAPHY;1998-01
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