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Determination of Organic Acids in Fermentation Broth of Spiramycin by High Performance Liquid Chromatography

LI You yuan 1, CHEN Chang hua 1, TAO Ping 2 (1.State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; 2.Instrument Analysis Center of Shanghai Jiaotong University, Shanghai, 200030,  
A method for determining organic acids in spiramycin fermentation broth by high performance liquid chromatography is described The operating conditions were Zorbax 300 SB C 18 column (5 μm,4 6 mm i.d.×15 cm) at 35 ℃, 0 01 mol/L phosphoric acid buffer solution (pH 2 32) and methanol as mobile phase at a flow rate of 0 6 mL/min and UV detection at 210 nm The relative standard deviations were 0 33%-0 10% and the recoveries were 99 95%-100 08% It's a simple, rapid and accurate method
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