Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Chromatography》 2003-03
Add to Favorite Get Latest Update

Studies on Determination and Pharmacokinetics of Rhubarb Anthraquinones in Rabbit Plasma by High Performance Liquid Chromatography

JIANG Xinhui1, ZHANG Dan2, CHEN Shujie2(1. Department of Pharmacy, Chongqing University of Medical Sciences, Chongqing 400016, China; 2. West China School of Pharmacy, Sichuan University, Chengdu 610044, China)  
A high performance liquid chromatographic method for the determination of rhubarb anthraquinones in rabbit plasma samples was established. The pretreatment method included the following steps: to hydrolyze rhubarb anthraquinones with 15% sulfuric acid in (70±2) ℃ water bath, to extract rhubarb anthraquinones with ethyl ether and then evaporate the ether layer to dryness, to reconstitute the residue with methanol and inject it into HPLC for analysis. Rhubarb anthraquinones and internal standard (1,8dihydroxy9,10anthraquinone) were separated on a μBondapak C18 column with methanolacetonitrile02 mol/L phosphate buffer of pH 28(27∶355∶375, v/v) as mobile phase and detected at 225 nm. The rhubarb anthraquinones and internal standard were separated. The calibration curves were linear within the range of 0099?0-2485?8 mg/L,0192?5-4811?0 mg/L, 0025?8 -0644?8 mg/L and 0045?1-1075?7 mg/L for aloeemodin, rhein, emodin and chrysophanol respectively, and the detection limits were 0002-0005 μg (S/N3) for all. The extraction recoveries of rhubarb anthraquinones were 5308%-6367% and analytical recoveries were 980%-1006% with the withinday RSD of 073%-086% and the betweenday RSD of 082%-173%. After oral administration of a single component preparation (extract solution of rhubarb) and compound preparation (WPY), the plasma concentrationtime curves of the two preparations fitted twocompartment model in rabbits. The method is simple, sensitive, accurate, precise and reproducible, and suitable for the study of rhubarb anthraquinones in rabbits. The AUC value and Cmax between single component and compound preparations had no significant difference (p005), and it showed that the other components had no effect for the absorption of rhubarb anthraquinones in rabbits.
【CateGory Index】: O657.7
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 6 Hits
1 CHAI Yi-feng, WU Yu-tian and LI Xiang(Department of Pharmaceutical Analysis, College of Pharmacy, Second Military Medical University, Guohe Road 325, Shanghai 200433), Fenxi Shiyanshi,2004,23(6):71~92;Pharmaceutical Analysis I[J];Analytical Laboratory;2004-06
2 KONG Liang~1, BAO Yongming~2, YU Zhiyuan~1, LI Wei~1, CHEN Xueguo~1, HU Lianghai~1, ZOU Hanfa~1(1. Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116023, China; 2. School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116024, China);Screening of Antineoplastic Components in Radix et Rhizoma Rhei Using Chromatographic Fingerprints[J];Chinese Journal of Chromatography;2005-05
3 MA Yue-ming,ZHAO Yang,XIE Hua,WANG Tian-ming,ZHU Shi-min,WU Yao-ping,ZHANG Ning(Laboratory of Pharmacokinetics,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China);Pharmacokinetics of anthraquinones in rats after intragastric gavage of Taohechengqi decoction[J];Chinese Pharmacological Bulletin;2005-10
4 ZHU Wei~(1), ZHANG Li~(2), WANG Xue-mei~(1), WANG Bao-xiu~(3), LI Xiao-ye~(2) (1. Institute of Integrated Traditional and Western Medicine of Peking University First Hospital, Beijing 100034, China; 2. Department of Chemistry, Fourth Military Medical University of China, Shanxi 710032, China;3. Department of Pharmacology, Zhejiang Pharmacology College, Zhejiang 315010,China);The pharmacokinetics of rhein in 12 healthy volunteers after oral administration of rhubarb extract[J];China Journal of Chinese Materia Medica;2005-18
5 Guo Jian,Li Min,Zhang Xiao-wei(Liaoning Center for Disease Control and Prevention,Shenyang 110005,China);Determination of three kinds of anthraquinone derivatives in health foods by HPLC[J];Chinese Journal of Health Laboratory Technology;2007-09
6 ZHU Wei, WANG Xue-mei Institute of Integrated Traditional Chinese and Western Medicine, the First Hospital, Peking University, Beijing (100034);Progress in Study on Mechanisms of Rhubarb in Treating Chronic Renal Failure[J];Chinese Journal of Integrated Traditional and Western Medicine;2005-05
【Citations】
Chinese Journal Full-text Database 3 Hits
1 Zhou Jihong;Yuan Yisheng and Yang Junwei(Nanjing General Hospital of Nanjing Command ,210002);HPTLC Determination of Three Anthraquinone Derivatives of Chinese Rhubarb in Prasma[J];CHINESE JOURNAL OF PHARMACEUTICAL ANALYSIS;1995-06
2 Zhang Na;Li Hua(Dept .of Biochemical Pharmaceuties, Shandong Medical University, Jinang 250012);Study on the Ovarian Cancer Cell Membrane Marker[J];PHARMACEUTICAL BIOTECHNOLOGY;1995-02
3 SUN Yang and CHEN Qiong-Hua (Division of Biochemistry, Nanjing College of Pharmacy, Nanjing);ISOLATION AND IDENTIFICATION OF METABOLITES OF PHYSCION IN RATS AND MICE IN VIVO AND IN VITRO[J];Acta Pharmaceutica Sinica;1986-10
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Li Laisheng 1,2 , Liu Min 1, Da Shilu *1 , Feng Yuqi 1 1(Department of Chemistry, Wuhan University, Wuhan 430072) 2(Analytical and Testing Center, Nanchang University, Nanchang 330047);The Chromatographic Behavior of Hydroxyanthroquinones from Rheum Palmatum L. on p-tert-Butyl-calix[8]arene-bonded Silica Gel Stationary Phase by High Performance Liquid Chromatography[J];Chinese Journal of Analytical Chemistry;2004-04
2 LI Lanfang, ZHANG Qinzeng, XIE Lijun,et al.Hebei Academy of Medical Sciences, Shijiazhuang 050021;Determination of emodin from tongmaihuoxueling capsules and study of its pharmacokinetics in plasma of rats[J];Hebei Medical Journal;2006-11
3 ;Contrast Study on the Content of Main Component of Cut Crude Drug of Pieplant and Its Mianjian Granule[J];Guiding Journal of TCM;2006-06
4 Zhao Feilang,Yuan Yisheng,Li Kaiwu Research Group of New Drugs,Jinling Hospital(Nanjing, 210002);Determination of five antraquinones in chinese Rhubuarbs and other samples[J];BULLETIN OF JINLING HOSPITAL;1999-02
5 DONG Yu,WANG Hong-wei,CHEN Chao-jun,ZHAO Guo-ding,GUAN Hai-bin(School of Pharmacology,Inner Mongolia Medical College,Inner Mongolia Autonomous Region 010059);Chemical compositions of Lignum Xanthoceratis[J];Journal of Beijing University of Traditional Chinese Medicine;2008-12
6 MA Tao,XIONG Chun-mei,GUO Ya-dong (Faculty of Pharmacy,Kunming Medical College,Yunnan Kunming 650031,China);Determination of Emogin and Chrysophanol in Rhenm L.by Rapid High Performance Liquid Chromatography[J];Journal of Kunming Teachers College;2005-04
7 TAN Xiao-hong,ZHANG Dan-shen,ZHANG Li,AN Fang,WANG Shu-hua,BAI Xue-mei,JIANG Hua(Department of Chemistry and Pharmacology,Hebei North University,Zhangjiakou 075029,Hebei,China);Determination of Chrysophanol in Rabbit Organs by RP-HPLC[J];Liaoning Journal of Traditional Chinese Medicine;2007-07
8 Zhang Na, Li Hua, Cao Jichao, Sun Shusan, Xu HongyanDeptarment of Biochemical Pharmaceutics, Shandong Medical University (Jinan 250012);Purification of Ovarian Cancer Cell Membrane Marker and Its Antibody[J];Chinese Journal of Biochemical Pharmaceutics;1996-03
9 KONG Liang~1, BAO Yongming~2, YU Zhiyuan~1, LI Wei~1, CHEN Xueguo~1, HU Lianghai~1, ZOU Hanfa~1(1. Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116023, China; 2. School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116024, China);Screening of Antineoplastic Components in Radix et Rhizoma Rhei Using Chromatographic Fingerprints[J];Chinese Journal of Chromatography;2005-05
10 TAN Xiao-hong,ZHANG Dan-shen~*,ZHANG Li,AN Fang,BAI Xue-mei,JIANG Hua(Department of Chemistry and Pharmacology,Hebei North University,Zhangjiakou,Hebei 075029,China);Determination of Chrysophanol in Rabbit Plasma by HPLC[J];LiShiZhen Medicine and Materia Medica Research;2006-07
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 HUANG Yu-MingIntegrate Ward, Suining People's Hospital, Suining 629000, China;Effect of Chinese medical herbal retention enema on renal function of patients with chronic renal failure[J];Journal of the Fourth Military Medical University;2007-23
2 LI Nan1,2,PAN Hong1,DING Shao-dong1,WANG Yun1 and CAO Yuhua1(1.College of Chemical and Material Engineering,Southern Yangtz university,Wuxi 214122;2.College of Life Science and Engineering,Shanxi University of Science and Technology,Xi′an 710021);Application on fingerprint of rhubarb by microemulsion capillary electrokinetic chromatography[J];Chinese Journal of Analysis Laboratory;2008-09
3 ;Clinical Study Progress on the Traditional Chinese Medicine for the Treatment of Chronic Renal Failure[J];Guiding Journal of TCM;2007-01
4 ZHANG Xiu-li,WANG Xin-ying,SHI Lan-xiang(College of Materials Science and Engineering,China Geology University,Beijing 100083,China);Determination of 7-amido acetoxy eicosanoic acid by ultraviolet spectrophotometric method[J];Chemical Engineer;2006-07
5 HUANG He-qin (People's Hospital Infection Section in Dafeng City of Jiangsu Province 224100,China);Azithromycin treatment of scrub typhus caused by the rhubarb effect of kidney damage[J];Journal of Liaoning University of Traditional Chinese Medicine;2008-10
6 YUAN Xiao-qing, MA Xiang-hua, DING Ya-qin, WANG Fang, SHEN Jie 1 (Department of Endocrinology,1Central Laboratory,the First Affiliated Hospital of NJMU,Nanjing 210029,China);Effects of Rhein on proliferation and differentiation of human preadipocytes[J];Acta Universitatis Medicinalis Nanjing(Natural Science);2009-02
7 YAO Li-mei1,ZHU Wei2(1.Guangdong Food and Drug Vocational College,Guangzhou 510520,China;2.Guangdong Hospital of Traditional Chinese Medicine Heart Center,Guangzhou 510120,China);The New Research Territory of TCM Pharmacokinetics——Population Pharmacokinetics/Pharmacodynamics[J];Lishizhen Medicine and Materia Medica Research;2008-06
8 ZHU Wei,QIU Xiao-hui(Preparation Laboratory of Traditional Chinese Medicine,Guangdong Hospital of Traditional Chinese Medicine,Guangzhou,Guangdong 510120,China);Simultaneous Determination of Anthraquinones in Rheum officinalis Baill Water Extract by RP-HPLC[J];Pharmacy Today;2009-06
9 ZHU Wei,RUAN Xin-ming,CHEN Ke-ji~1Heart Center of Guangdong Hospital of Traditional Chinese Medicine,Guangzhou 510106,Guangdong,China;~1 Xiyuan Hospital of China Academy of Traditional Chinese medicine,Beijing 100091,China;Effect of gender difference on pharmacokinetics of rhein healthy volunteers after oral administration of rhubarb extract[J];Chinese Journal of Clinical Pharmacology and Therapeutics;2006-02
10 CHEN Wei-ling (Department of Kidney Medicine, Huizhou Central People's Hospital, Guangdong Province, Huizhou 516001, China);The curative effects comparison of the two methods in the treatment of chronic renal failure[J];China Medical Herald;2008-36
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 HSU GIN-YU, SUN CHING-CHIN, CHEN CHIUNG-HUA AND WU WU-TUNG (Department of Biochemistry, Nanking College of Pharmacy, Nanking);STUDIES ON CHINESE RHUBARB X. METABOLIC TRANSFORMATIONS OF ANTHAQUINONE DERIVATIVES[J];生物化学与生物物理学报;1966-02
2 Zhou Jihong;Yuan Yisheng and Yang Junwei(Nanjing General Hospital of Nanjing Command ,210002);HPTLC Determination of Three Anthraquinone Derivatives of Chinese Rhubarb in Prasma[J];CHINESE JOURNAL OF PHARMACEUTICAL ANALYSIS;1995-06
3 CHEN CHIUNG-HUA CHENG WU-FEI SU HSUEH-LIANG LAI WEI-SHENG (Departments of Biochemistry and Microbiology,Tientsin Medical College,Tientsin);STUDIES ON CHINESE RHUBARB——Ⅰ.PRELIMINARY STUDY ON THE ANTIBACTERIAL ACTIVITY OF ANTHRAQUINONE DERIVATIVES OF CHINESE RHUBARB(RHEUM PALMATUM L.)[J];Acta Pharmaceutica Sinica;1962-12
4 Zheng Zhong Ji Zhongmin (Department of Pathology);The Specificity and Significance of Lectin Receptors in Breast Carcinoma[J];;1989-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved